抗体

MOUSE抗猪CD8乙CHAIN:异硫氰酸荧光素

MBS224819

0.1毫克

505美元

单克隆

小鼠

异硫氰酸荧光素

[PPT23]

流式细胞仪, 流式细胞仪

抗体

MOUSE抗猪CD8乙CHAIN:异硫氰酸荧光素

[CD8乙链]

[CD8乙链;T细胞表面的糖蛋白CD8乙链;T细胞表面的糖蛋白CD8乙链;CD8b分子;CD_antigen:CD8b]

[CD8]

[CD8B;CD8B;LY3;P37;LEU2;LYT3;CD8B1;CD8B1]

CD8B_HUMAN

单克隆

IgG1

[PPT23]

小鼠

猪

30

CD8乙

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

IgG浓度0.1毫克/毫升

Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch

流式细胞仪(FC/流式细胞仪)

Flow Cytometry: Neat- 1/10. Use 10ul of the suggested working dilution to label 1x10 6 cells in 100ul. Where the product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their system using appropriate negative/positive controls.

流式细胞仪(FC/流式细胞仪)

流式细胞仪(FC/流式细胞仪)

流式细胞仪(FC/流式细胞仪)

Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Preservative Stabilisers: 0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin . Buffer Solution: Phosphate buffered saline

Immunogen: Porcine thymus membrane lysate. Fusion partners: Lymph node cells from immunized BALB/c mice were fused with cells of the NSO myeloma cell line

Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen. Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3 + , CD4 - , CD8 hi and as such defines this antibody as a specific marker of porcine alpha/beta T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22.

4261577

AAD13877.1

22,237 Da

Adaptive Immune System Pathway (1269171); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Disease Pathway (1268854); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); HIV Infection Pathway (1269057); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]

CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: early endosome membrane; external side of plasma membrane; extracellular region; integral to plasma membrane; plasma membrane; T cell receptor complex. Molecular Function: coreceptor activity; MHC class I protein binding; protein binding. Biological Process: adaptive immune response; immune response; regulation of defense response to virus by virus; regulation of immune response; T cell activation; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction

0.1毫克

505美元