抗体

Anti-TGFBR2 Antibody (aa13-40) IHC-plus

MBS2400063

0.2毫升

530美元

多克隆

兔

未共轭

人类, 小鼠

免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

抗体

Anti-TGFBR2 Antibody (aa13-40) IHC-plus

[TGFBR2]

[Rabbit Polyclonal to Human TGFBR2; Human TGFBR2; TGFBR2; AAT3; FAA3; HNPCC6; LDS2B; MFS2; TGF-beta receptor type-2; TGF-beta RII; TGFBRII; TGF-beta type II receptor; TGFbeta-RII; TbetaR-II; TGF beta Receptor II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGFR-2; LDS1B; RIIC; TAAD2]

[TGF-beta receptor type-2 isoform B; TGF-beta receptor type-2; TGF-beta receptor type-2; transforming growth factor beta receptor II; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II]

[TGFBR2]

[TGFBR2; TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII; TGFR-2; TGF-beta receptor type II; TbetaR-II]

TGFR2_HUMAN

多克隆

兔

小鼠, 人类

567

This TGFBR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human TGFB2.

蛋白质A纯化

PBS, 0.09% 叠氮化钠

保持冷冻状态2-8°C长达6个月。 长时间储存于-20°C

免疫组化-石蜡(免疫组化-P), 免疫荧光(IF), 免疫印迹(免疫印迹)

IHC-P (1:100). IF (1:10 - 1:50). WB (1:1000). (Optimal dilution to be determined by the researcher.)

免疫组化(免疫组化)

免疫组化(免疫组化)

免疫荧光(IF)

免疫印迹(免疫印迹)

TGFBR2 Antibody western blot of mouse lung tissue lysates (35 ug/lane). The TGFBR2 antibody detected the TGFBR2 protein (arrow).

Immunogen Species: TGFBR2 antibody was raised against Human. Epitope: aa13-40. Target Species: Human. Family/Subfamily: Protein Kinase/ Activin/TGFb

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip . Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

67782324

NP_003233.4

NM_003242.5

P37173

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); ALK1 Signaling Events Pathway (137968); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (83106)

This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]

TGFBR2: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Binds to DAXX. Defects can cause esophageal cancer. Protein type: Kinase, protein; Oncoprotein; Protein kinase, TKL; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; TKL group; STKR family; Type2 subfamily. Chromosomal Location of Human Ortholog: 3p22. Cellular Component: caveola; cytosol; external side of plasma membrane; integral to membrane; lipid raft; plasma membrane; receptor complex. Molecular Function: ATP binding; glycosaminoglycan binding; metal ion binding; mitogen-activated protein kinase kinase kinase binding; protein binding; receptor signaling protein serine/threonine kinase activity; SMAD binding; transforming growth factor beta binding; transforming growth factor beta receptor activity; transforming growth factor beta receptor activity, type II; transmembrane receptor protein serine/threonine kinase activity. Biological Process: activation of protein kinase activity; aging; apoptosis; blood vessel development; brain development; common-partner SMAD protein phosphorylation; embryo implantation; embryonic cranial skeleton morphogenesis; embryonic hemopoiesis; gastrulation; gut development; heart development; in utero embryonic development; lens development in camera-type eye; myeloid dendritic cell differentiation; negative regulation of cardiac muscle cell proliferation; negative regulation of transforming growth factor beta receptor signaling pathway; Notch signaling pathway; organ regeneration; palate development; patterning of blood vessels; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of angiogenesis; positive regulation of B cell tolerance induction; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of NK T cell differentiation; positive regulation of skeletal muscle regeneration; positive regulation of smooth muscle cell proliferation; positive regulation of T cell tolerance induction; positive regulation of tolerance induction to self antigen; protein amino acid phosphorylation; receptor-mediated endocytosis; regulation of cell proliferation; regulation of gene expression; response to drug; response to estrogen stimulus; response to glucose stimulus; response to mechanical stimulus; response to nutrient; smoothened signaling pathway; transforming growth factor beta receptor signaling pathway; vasculogenesis; wound healing. Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 6; Esophageal Cancer; Loeys-dietz Syndrome 2

0.2毫升

530美元