产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Anti-CD20 Antibody IHC-plus
目录 :
MBS2400340
规格 :
0.05毫克
价格 :
530美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法, 免疫组化-石蜡切片
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS2400340 图像 1
Mouse Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
产品信息
目录号 :
MBS2400340
产品类型 :
抗体
产品全称 :
Anti-CD20 Antibody IHC-plus
产品简称 :
[CD20]
产品名称同义词 :
[Rabbit Polyclonal (IgG) to Mouse CD20; Mouse CD20; MS4A1; B-lymphocyte antigen CD20; B1; Bp35; CD20; CD20 antigen; CD20 receptor; LEU-16; CVID5]
其他名称 :
[B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; membrane spanning 4-domains A1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20]
产品基因名称 :
[CD20]
产品基因名称同义词 :
[跨膜结构域4超家族成员1]
其他基因名称 :
[跨膜结构域4超家族成员1;跨膜结构域4超家族成员1;B1;S7;Bp35;CD20;CVID5;MS4A2;LEU-16;CD20]
UniProt数据库进入名 :
CD20_HUMAN
克隆性 :
多克隆
抗体亚型 :
IgG
宿主 :
反应物种 :
小鼠
序列长度 :
297
特异性 :
小鼠CD20
纯度 :
Caprylic acid and ammonium sulfate precipitation
形式 :
PBS, pH值7.4, 0.03% ProClin防腐剂300, 50% 甘油
浓度 :
1毫克/毫升
储存稳定性 :
分成小份儿保存在-20°C或-80°C。 避免冻融循环
检测过的应用 :
免疫组化(免疫组化)石蜡, 免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA)
应用笔记 :
IHC-P (5 ug/ml) . Optimal dilution to be determined by the researcher.
image1头 :
免疫组化-石蜡(免疫组化)
其它信息1 :
Immunogen: CD20 antibody was raised against recombinant mouse B-lymphocyte antigen CD20 protein. Immunogen Species: CD20 antibody was raised against Mouse. Antigen Type: Recombinant protein. Target: CD20
其他信息2 :
Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip. Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
产品描述 :
MS4A1 Antibody, B-lymphocyte antigen CD20 Antibody, B1 Antibody, Bp35 Antibody, CD20 Antibody, CD20 antigen Antibody, CD20 receptor Antibody, LEU-16 Antibody, CVID5 Antibody Description: CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members.
NCBI GI登录号 :
23110989
NCBI登录号 :
NP_690605.1
NCBI基因登录号 :
NM_152866.2
UniProt数据库登录号 :
P11836
NCBI信号通路 :
Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)
NCBI总结 :
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell surface. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: external side of plasma membrane; extracellular space; integral to plasma membrane. Molecular Function: epidermal growth factor receptor binding; protein binding. Biological Process: B cell proliferation; humoral immune response. Disease: Immunodeficiency, Common Variable, 5
尺寸1 :
0.05毫克
价格1 :
530美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。