抗体

兔多抗人类HSP90AA1/Hsp90

MBS240566

0.05毫克

495美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠, 鸡

免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法

抗体

兔多抗人类HSP90AA1/Hsp90

HSP90AA1/Hsp90

Anti-HSP90AA1 / Hsp90 Antibody (aa289-300) IHC-plus; HSP90AA1; EL52; HSP86; Hsp89; HSP89A; HSPC1; Heat shock 86 kDa; Hsp90; HSP90A; HSP90N; HSPCA; HSPCAL4; HSPN; LAP2; HSP 86; HSPCAL1; Human HSP90AA1; Hsp90

heat shock protein HSP 90-alpha isoform 2; Heat shock protein HSP 90-alpha; heat shock protein HSP 90-alpha; HSP 86; heat shock 86 kDa; LPS-associated protein 2; heat shock 90kD protein 1, alpha; heat shock 90kDa protein 1, alpha; renal carcinoma antigen NY-REN-38; heat shock 90kD protein, alpha-like 4; epididymis luminal secretory protein 52; heat shock 90kD protein 1, alpha-like 4; lipopolysaccharide-associated protein 2; heat shock protein 90kDa alpha (cytosolic), class A member 1; Heat shock 86 kDa; HSP 86; HSP86; Lipopolysaccharide-associated protein 2; LAP-2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38

HSP90AA1

Hsp90

HSP90AA1; HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4; HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2

HS90A_HUMAN

多克隆

兔

Chicken, Drosophila, Human, Monkey, Mouse, Rat

732

氨基酸289-300人的Hsp90蛋白

Immunoaffinity Purified

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2, 0.01% 叠氮化钠

1毫克/毫升

Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

免疫组化(免疫组化-石蜡), 免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA)

ELISA (1:5000 - 1:25000), IHC-P (2.5 ug/ml), WB (1:500 - 1:2500). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.

Target Species: Human. Immunogen Description: Synthetic peptide from human HSP90AA1 / Hsp90. Usage Summary: Immunohistochemistry: MBS240566 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS240566 was determined to be 2.5 ug/ml. Summary: Hsp90 is a member of the heat shock protein 90 family that functions as a molecular chaperone and has ATPase activity. Hsp90 family proteins are highly conserved between isoforms and species. Several signal transduction pathways depend on Hsp90 function including erbB2, steroid hormone receptors (such as androgen, progesterone, glucocorticoid, and aryl-hydrocarbon), and hypoxia sensing (Hif1 alpha). Recent reports show that tumor cells are more sensitive to Hsp90 inhibition and that Hsp90 from tumor cells is more sensitive to small molecule inhibitors (eg 17AAG). The mechanism of this differential sensitivity of normal versus tumor Hsp90 is not known (although mutation has been ruled out). One possible mechanism may be differences in post-translational modification of tumor Hsp90. Hsp90 is a cytoplasmic protein that forms a homodimer in vivo, and interacts with TOM34, AHSA1, HDAC6 and SMYD3 .

Immunogen Type: Synthetic peptide. Immunogen: HSP90AA1 / Hsp90 antibody was raised against synthetic peptide from human HSP90AA1 / Hsp90. Antigen Modification: aa289-300

HSP90AA1 is a member of the group of proteins produced by cells to deal with stress such as elevated temperature. It is known to chaperone a key subset of signaling proteins and is necessary for malignant transformation. HSP90 is subject to an array of post translational modifications that affect its function including acetylation.

154146191

NP_005339.3

NM_005348.3

P07900

98,161 Da

AhR Pathway (755436); Anchoring Of The Basal Body To The Plasma Membrane Pathway (1127503); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Assembly Of The Primary Cilium Pathway (1127502); Attenuation Phase Pathway (1127556); Axon Guidance Pathway (105688); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765)

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

HSP90A: a molecular chaperone of the heat shock protein 90 family. Has ATPase activity. Known to interact with a wide variety of proteins including steroid hormone receptors, neuropeptide Y, FKBP51/54, and FKBP52. G protein-coupled receptor kinases are stabilized by interacting with HSP 90. Hsp70 and Hsp90 promote tau solubility and tau binding to microtubules, reducing insoluble tau phosphorylation of tau. Protein type: Heat shock protein; Chaperone. Chromosomal Location of Human Ortholog: 14q32.33. Cellular Component: nucleoplasm; mitochondrion; membrane; cytoplasm; plasma membrane; melanosome; extracellular region; nucleus; cytosol. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; TPR domain binding; nitric-oxide synthase regulator activity; unfolded protein binding; ATPase activity; nucleotide binding; ATP binding. Biological Process: axon guidance; receptor-mediated endocytosis; positive regulation of nitric oxide biosynthetic process; organelle organization and biogenesis; signal transduction; nitric oxide metabolic process; protein import into mitochondrial outer membrane; response to unfolded protein; mitochondrial transport; innate immune response; protein refolding; mitotic cell cycle; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; chaperone-mediated protein complex assembly; G2/M transition of mitotic cell cycle

0.05毫克

495美元