产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Mouse Monoclonal [clone 15.2] (IgG1) to Human ICAM1 / CD54
目录 :
MBS245394
规格 :
0.05毫克
价格 :
530美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
[15.2]
反应物种 :
人类
应用 :
免疫组化, 免疫沉淀, 流式细胞仪, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS245394 图像 1
Anti-ICAM-1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
图像 2 :
MyBioSource MBS245394 图像 2
Anti-ICAM-1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
产品信息
目录号 :
MBS245394
产品类型 :
抗体
产品全称 :
Mouse Monoclonal [clone 15.2] (IgG1) to Human ICAM1 / CD54
产品简称 :
[细胞间粘附分子1/CD54]
产品名称同义词 :
[Anti-ICAM1 / CD54 Antibody (clone 15.2) IHC-plus; ICAM1; BB2; CD54 antigen; CD54; ICAM-1; p3.58; Human ICAM1; CD54]
其他名称 :
[intercellular adhesion molecule 1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; CD_antigen: CD54]
产品基因名称 :
[细胞间粘附分子 1]
产品基因名称同义词 :
[CD54]
其他基因名称 :
[细胞间粘附分子1;细胞间粘附分子1;BB2;CD54;P3.58;ICAM-1]
UniProt数据库进入名 :
ICAM1_HUMAN
克隆性 :
单克隆
抗体亚型 :
IgG1
克隆 :
[15.2]
宿主 :
小鼠
反应物种 :
人类, 猪
序列长度 :
532
特异性 :
Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Clone 15.2 is reported to block CD54 function.
纯度 :
蛋白质G纯化
形式 :
PBS, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
储存稳定性 :
+4°C or -20°C, Avoid repeated freezing and thawing.
检测过的应用 :
免疫组化(免疫组化-石蜡;免疫组化-冷冻), 免疫沉淀(免疫沉淀), 流式细胞仪(FC/流式细胞仪)
应用笔记 :
IHC - Paraffin (10 ug/ml). IHC - Frozen. Immunoprecipitation. Flow Cytometry (1:50 - 1:100). Immunohistochemistry: MBS245394 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245394 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul.
image1头 :
免疫组化(免疫组化)
image2头 :
免疫组化(免疫组化)
其它信息1 :
Immunogen Description: Raised against Human monocytes. Immunogen Species: Raised against Human. Antigen Type: Cells
其他信息2 :
Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip
产品种类 :
Family: ICAM
产品描述 :
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. Immunohistochemistry: MBS245394 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245394 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul.
NCBI GI登录号 :
167466198
NCBI登录号 :
NP_000192.2
NCBI基因登录号 :
NM_000201.2
UniProt数据库登录号 :
P05362
NCBI分子量 :
57,825 Da
NCBI信号通路 :
Adaptive Immune System Pathway (366160); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine Signaling In Immune System Pathway (366171); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Extracellular Matrix Organization Pathway (576262); Glucocorticoid Receptor Regulatory Network Pathway (138014)
NCBI总结 :
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta. Protein type: Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: extracellular space; cell surface; focal adhesion; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane. Molecular Function: viral receptor activity; integrin binding; protein binding; transmembrane receptor activity; receptor activity. Biological Process: extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; leukocyte adhesion; cellular response to nutrient levels; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; response to drug; regulation of cell adhesion; virion attachment, binding of host cell surface receptor; regulation of immune response; negative regulation of calcium ion transport; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to copper ion; positive regulation of actin filament polymerization; positive regulation of vasoconstriction; cell adhesion mediated by integrin; adhesion to host; response to ionizing radiation; leukocyte migration. Disease: Malaria, Susceptibility To
尺寸1 :
0.05毫克
价格1 :
530美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。