抗体

Mouse Monoclonal [clone B2M-01] (IgG2a) to Human B2M / Beta 2 Microglobulin

MBS246681

0.05毫克

530美元

单克隆

小鼠

未共轭

[B2M-01]

人类, 狗, 鸡, 牛

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪, 流式细胞仪, 放射免疫分析法, 酶免疫法

抗体

Mouse Monoclonal [clone B2M-01] (IgG2a) to Human B2M / Beta 2 Microglobulin

[B2M/bata2微球蛋白]

[Anti-B2M / Beta 2 Microglobulin Antibody (clone B2M-01) IHC-plus; B2M; Beta 2 Microglobulin; Beta-2-microglobulin; Beta-2-microglobin; Human B2M; Beta 2 Microglobulin]

[beta-2-microglobulin; Beta-2-microglobulin; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules; beta-2-microglobulin]

[B2M]

[B2M;B2M]

B2 MG_HUMAN

单克隆

IgG2a

[B2M-01]

小鼠

Reactivity: Human (tested or 100% immunogen sequence identity). Non-Reactivity: Mouse, Bovine, Dog, Rabbit, Chicken

119

reacts with beta2-microglobulin (b2M) associated with cell-surfaceMHC Class I molecules and other membrane antigens as well as with solublebeta2-microglobulin. b2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes,thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It isabsent on erythrocytes.

纯化

PBS含15 mM在叠氮化钠, pH值7.4

1毫克/毫升

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

免疫组化(免疫组化-石蜡), 免疫印迹(免疫印迹), 免疫沉淀(免疫沉淀), 流式细胞仪(FC/流式细胞仪), 酶联免疫吸附测定(EIA), 放射免疫分析法

ELISA, Flo, IHC-P (1:50), IP, RIA, WB (2 - 4 ug/ml). Usage: For use in immunoprecipitation, this antibody coprecipitates b2-microglobulin (12 kDa) and HLA antigens(45 kDa) from the lysates of human lymphoid cells. Recommend U937 human histiocytic lymphoma cell line orRAJI human Burkitt lymphoma cell line as postitive control and EL4 mouse lymphoblastic lymphoma cell line as negative control for Western blot. Reducing and non-reducing conditions. Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Recommend peripheral blood lymphocytes as positve control and DAUDI human Burkitt lymphoma cell line, or erythrocytes as negative control for flow cytometry. Recommend kidney glomeruli as postive control for IHC. For RIA, use at an assay dependent concentration. The dissociation constant of the antibody soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.

免疫组化(免疫组化)

Target Species: Human. Immunogen Description: Purified human beta2-microglobulin

Immunogen Type: Purified protein. Immunogen: B2M / Beta 2 Microglobulin antibody was raised against purified human beta2-microglobulin

B2M / Beta 2 Microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

4757826

NP_004039.1

NM_004048.2

P61769

13,715 Da

Adaptive Immune System Pathway (366160); Amyloids Pathway (366238); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cytokine Signaling In Immune System Pathway (366171); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550)

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 15q21.1. Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; early endosome membrane; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region; MHC class I protein complex; external side of plasma membrane. Molecular Function: identical protein binding; protein binding. Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent. Disease: Hypoproteinemia, Hypercatabolic

0.05毫克

530美元