抗体

Mouse Monoclonal [clone 1H6] (IgG1) to Human PECAM-1 / CD31

MBS249056

0.05毫升

530美元

单克隆

小鼠

未共轭

[1H6]

人类, 狗

免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 流式细胞仪

抗体

Mouse Monoclonal [clone 1H6] (IgG1) to Human PECAM-1 / CD31

[PECAM-1/CD31]

[PECAM1;CD31;CD31抗原;EndoCAM;PECA1;PECAM-1;GPIIA]

[血小板内皮细胞 粘连分子;血小板内皮细胞 粘连分子;血小板内皮细胞 粘连分子;CD31抗原;血小板/内皮细胞 粘连分子1;EndoCAM;GPIIA';PECA1;CD_antigen:CD31]

[PECAM-1]

[CD31]

[PECAM1;PECAM1;CD31;PECA1;GPIIA';PECAM-1;endoCAM;CD31/EndoCAM;PECAM-1]

PECA1_HUMAN

单克隆

IgG1

[1H6]

小鼠

人类, 狗

人类CD31

Immunoaffinity purified from tissue culture supernatant

PBS, pH值7.3, 0.02% 叠氮化钠, 50% 甘油, 1% BSA

1.2毫克/毫升

存放在-20°C。 避免冻融循环

免疫组化-石蜡(免疫组化), 免疫荧光(IF), 免疫印迹(免疫印迹), 流式细胞仪(FC/流式细胞仪)

IHC - Paraffin (20 ug/ml). Immunofluorescence (1:100). Western Blot (1:500 - 1:2000). Flow Cytometry (1:100). (Optimal dilution to be determined by the researcher.)

免疫组化(免疫组化)

免疫荧光(IF)

流式细胞仪(FC/流式细胞仪)

免疫印迹(免疫印迹)

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-PECAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

免疫印迹(免疫印迹)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.

Immunogen Species: PECAM-1 / CD31 antibody was raised against Human.

. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip.

CD31 (PECAM1) is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

110347451

NP_000433.3

NM_000442.3

P16284

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028); Integrin Cell Surface Interactions Pathway (106110); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Malaria Pathway (152665); Malaria Pathway (152657)

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 17q23.3. Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction. Molecular Function: protein binding. Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; signal transduction; cell adhesion; leukocyte migration; phagocytosis

0.05毫升

530美元