产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
Anti-PTGS2 / COX2 / COX-2 Antibody (C-Terminus) IHC-plus
目录 :
MBS249808
规格 :
0.05毫升
价格 :
530美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS249808 图像 1
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
产品信息
目录号 :
MBS249808
产品类型 :
抗体
产品全称 :
Anti-PTGS2 / COX2 / COX-2 Antibody (C-Terminus) IHC-plus
产品简称 :
[PTGS2/COX2/COX-2]
产品名称同义词 :
[Rabbit Polyclonal to Human PTGS2 / COX2 / COX-2; Human PTGS2 / COX2 / COX-2; Cyclooxygenase-2; Cyclooxygenase 2b; HCox-2; GRIPGHS; PGH synthase 2; PGHS-2; PHS II; PHS-2; Prostaglandin H2 synthase 2; PGG/HS; Prostaglandin G/H synthase 2]
其他名称 :
[prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2; prostaglandin G/H synthase 2; prostaglandin-endoperoxide synthase 2; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2]
产品基因名称 :
[PTGS2/COX2/COX-2]
其他基因名称 :
[PTGS2; PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; COX2; COX-2; PGH synthase 2; PGHS-2]
UniProt数据库进入名 :
PGH2_HUMAN
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠, 牛, 猪, 羊
序列长度 :
604
特异性 :
Recognizes endogenous levels of PGHS-2 protein.
纯度 :
Immunoaffinity purified
形式 :
PBS, pH值7.3, 0.01% 叠氮化钠, 30% 甘油
浓度 :
1毫克/毫升
储存稳定性 :
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
检测过的应用 :
免疫组化(免疫组化)石蜡, 免疫细胞化学(免疫细胞化学), 免疫荧光(IF), 免疫印迹(免疫印迹)
应用笔记 :
IHC-Paraffin (1:100). ICC(1:100-1:500). Immunofluorescence. Western Blot (1:500-1:1000). Optimal dilution to be determined by the researcher.
image1头 :
免疫组化-石蜡(免疫组化)
其它信息1 :
Immunogen species: PTGS2/COX2/COX-2 antibody was raised against Human. Immunogen : PTGS2 / COX2 / COX-2 antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human PGHS-2. Antigen Type: Synthetic peptide. Note: During shipment, small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical and toxicological properties of this material have not been throughly investigated. Standard laboratory Practices should be followed. Avoid sking and eye contact, inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plumbing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent acumulation.
其他信息2 :
Epitope: C-Terminus. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip
产品描述 :
PTGS2 Antibody, COX-2 Antibody, Cyclooxygenase-2 Antibody, COX2 Antibody, Cyclooxygenase 2b Antibody, HCox-2 Antibody, GRIPGHS Antibody, PGH synthase 2 Antibody, PGHS-2 Antibody, PHS II Antibody, PHS-2 Antibody, Prostaglandin H2 synthase 2 Antibody, PGG/HS Antibody, Prostaglandin G/H synthase 2 Antibody Description: Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis.
NCBI GI登录号 :
4506265
NCBI登录号 :
NP_000954.1
NCBI基因登录号 :
NM_000963.3
UniProt数据库登录号 :
P35354
NCBI信号通路 :
Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (1270087); Arachidonic Acid Metabolism Pathway (366); C-MYB Transcription Factor Network Pathway (138073); C20 Prostanoid Biosynthesis Pathway (545299); C20 Prostanoid Biosynthesis Pathway (908107); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237)
NCBI总结 :
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]
UniProt数据库总结 :
COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family. Protein type: Oxidoreductase; EC 1.14.99.1; Lipid Metabolism - arachidonic acid. Chromosomal Location of Human Ortholog: 1q25.2-q25.3. Cellular Component: caveola; cytoplasm; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; neuron projection; nucleus; protein complex. Molecular Function: arachidonate 15-lipoxygenase activity; enzyme binding; heme binding; lipid binding; metal ion binding; peroxidase activity; prostaglandin-endoperoxide synthase activity; protein binding; protein homodimerization activity. Biological Process: angiogenesis; arachidonic acid metabolic process; bone mineralization; brown fat cell differentiation; cell motility; cyclooxygenase pathway; decidualization; embryo implantation; hair cycle; inflammatory response; learning; lipoxygenase pathway; memory; NAD metabolic process; negative regulation of calcium ion transport; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of smooth muscle contraction; negative regulation of synaptic transmission, dopaminergic; nicotinamide metabolic process; ovulation; positive regulation of apoptosis; positive regulation of fever; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of prostaglandin biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle contraction; positive regulation of synaptic plasticity; positive regulation of synaptic transmission, glutamatergic; positive regulation of vasoconstriction; prostaglandin biosynthetic process; prostaglandin metabolic process; regulation of blood pressure; regulation of inflammatory response; response to drug; response to estradiol stimulus; response to fructose stimulus; response to glucocorticoid stimulus; response to lipopolysaccharide; response to lithium ion; response to manganese ion; response to oxidative stress; response to vitamin D; sensory perception of pain; vitamin metabolic process; water-soluble vitamin metabolic process
尺寸1 :
0.05毫升
价格1 :
530美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。