产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
小鼠ApoB100(载脂蛋白B100)酶联免疫吸附试剂盒
目录 :
MBS2502404
规格 :
24-Strip-Wells
价格 :
190美元
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS2502404 图像 1
产品信息
目录号 :
MBS2502404
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
小鼠ApoB100(载脂蛋白B100)酶联免疫吸附试剂盒
产品简称 :
[ApoB100]
其他名称 :
[载脂蛋白b-100;载脂蛋白b-100;载脂蛋白b-100;载脂蛋白B-100;载脂蛋白B 100;载脂蛋白B 48;载脂蛋白b(包括老化(x)抗原);载脂蛋白B48;突变体载脂蛋白B 100;载脂蛋白b]
产品基因名称 :
[ApoB100]
其他基因名称 :
[载脂蛋白B;载脂蛋白B;FLDB;LDLCQ4;载脂蛋白B-100;载脂蛋白B-48]
UniProt数据库进入名 :
APOB_HUMAN
反应物种 :
小鼠
序列长度 :
4563
特异性 :
This kit recognizes Mouse ApoA1 in samples. No significant cross-reactivity or interference between Mouse ApoA1 and analogues was observed.
储存稳定性 :
Store at 4 degree C.
image1头 :
Typical Testing Data/Standard Curve (for reference only)
其它信息1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 7.81-500ng/mL. Sensitivity: 4.69ng/mL.
产品描述 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse ApoA1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ApoA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ApoA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse ApoA1. You can calculate the concentration of Mouse ApoA1 in the samples by comparing the OD of the samples to the standard curve.
NCBI GI登录号 :
105990532
NCBI登录号 :
NP_000375.2
NCBI基因登录号 :
NM_000384.2
NCBI分子量 :
515,605 Da
NCBI信号通路 :
Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Cell Surface Interactions At The Vascular Wall Pathway (106062); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA1 Transcription Factor Network Pathway (137979); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (106028); LDL-mediated Lipid Transport Pathway (106159)
NCBI总结 :
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA- UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. Protein type: Secreted; Secreted, signal peptide; Carrier. Chromosomal Location of Human Ortholog: 2p24-p23. Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; intracellular membrane-bound organelle; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol; actin cytoskeleton; chylomicron; cell soma; cytoplasm; plasma membrane; endosome membrane. Molecular Function: heparin binding; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding. Biological Process: phototransduction, visible light; lipoprotein catabolic process; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to selenium ion; triacylglycerol catabolic process; retinoid metabolic process; transmembrane transport; receptor-mediated endocytosis; nervous system development; cholesterol metabolic process; cholesterol transport; in utero embryonic development; regulation of cholesterol biosynthetic process; response to virus; cholesterol efflux; lipoprotein metabolic process; sperm motility; cholesterol homeostasis; fertilization; lipoprotein transport; lipoprotein biosynthetic process; artery morphogenesis; spermatogenesis; blood coagulation; triacylglycerol mobilization; leukocyte migration. Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1
尺寸1 :
24-Strip-Wells
价格1 :
190美元
尺寸2 :
48-Strip-Wells
价格2 :
310
size3 :
96-Strip-Wells
价格3 :
355
size4 :
5x96-Strip-Wells
price4 :
1435
size5 :
10x96-Strip-Wells
price5 :
2805
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。