ELISA/assay

人类过氧化物酶体增殖物激活受体γ(过氧化物酶体增殖物激活受体γ)酶联免疫吸附试剂盒

MBS2503174

48-Strip-Wells

410美元

酶联免疫吸附试剂盒

人类过氧化物酶体增殖物激活受体γ(过氧化物酶体增殖物激活受体γ)酶联免疫吸附试剂盒

过氧化物酶体增殖物激活受体γ

peroxisome proliferator-activated receptor gamma isoform 1; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated nuclear receptor gamma variant 1; peroxisome proliferator-activated receptor gamma; Nuclear receptor subfamily 1 group C member 3

过氧化物酶体增殖物激活受体γ

过氧化物酶体增生物激活受体gamma;过氧化物酶体增生物激活受体gamma;GLM1;CIMT1;NR1C3;PPARG1;PPARG2;PPARgamma;NR1C3;过氧化物酶体增殖物激活受体γ

PPARG_HUMAN

人类

477

This assay has high sensitivity and excellent specificity for detection of Human PPAR-gamma. No significant cross-reactivity or interference between Human PPAR-gamma and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PPAR-gamma and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156--10ng/mL. Sensitivity: 0.094ng/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PPAR-gamma in human serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-gamma. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PPAR-gamma and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PPAR-gamma, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PPAR-gamma. You can calculate the concentration of Human PPAR-gamma in the samples by comparing the OD of the samples to the standard curve.

116284370

NP_619726.2

NM_138712.3

P37231

21,580 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198832); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Developmental Biology Pathway (477129); Energy Metabolism Pathway (198907); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Huntington's Disease Pathway (83100)

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 3p25. Cellular Component: nucleoplasm; perinuclear region of cytoplasm; nucleus; cytosol. Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; retinoid X receptor binding; arachidonic acid binding; protein binding; enzyme binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; prostaglandin receptor activity; sequence-specific DNA binding; estrogen receptor binding; steroid hormone receptor activity; chromatin binding; transcription factor activity. Biological Process: positive regulation of transcription, DNA-dependent; heart development; negative regulation of collagen biosynthetic process; cell maturation; rhythmic process; lipid homeostasis; glucose homeostasis; response to lipid; response to vitamin A; response to caffeine; positive regulation of oligodendrocyte differentiation; placenta development; long-chain fatty acid transport; organ regeneration; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; negative regulation of telomerase activity; cellular response to insulin stimulus; lipoprotein transport; response to estrogen stimulus; response to mechanical stimulus; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; positive regulation of transcription factor activity; steroid hormone mediated signaling; positive regulation of phagocytosis, engulfment; negative regulation of transcription, DNA-dependent; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; epithelial cell differentiation; regulation of blood pressure; response to nutrient; caspase activation; transcription initiation from RNA polymerase II promoter; response to retinoic acid; G-protein coupled receptor protein signaling pathway; response to low density lipoprotein stimulus; white fat cell differentiation; positive regulation of fatty acid oxidation; innate immune response; gene expression; lipid metabolic process; response to cold; negative regulation of cell growth. Disease: Obesity; Lipodystrophy, Familial Partial, Type 3; Carotid Intimal Medial Thickness 1; Diabetes Mellitus, Noninsulin-dependent

48-Strip-Wells

410美元

96-Strip-Wells

490