ELISA/assay

Rat PPAR-alpha (Peroxisome Proliferators Activator Receptors alpha) ELISA Kit

MBS2504779

24-Strip-Wells

215美元

酶联免疫吸附试剂盒

Rat PPAR-alpha (Peroxisome Proliferators Activator Receptors alpha) ELISA Kit

[过氧化物酶体增殖物激活受体α]

[peroxisome proliferator-activated receptor alpha; Peroxisome proliferator-activated receptor alpha; peroxisome proliferator-activated receptor alpha; PPAR-alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3; peroxisome proliferator-activated receptor alpha; Nuclear receptor subfamily 1 group C member 1]

[过氧化物酶体增殖物激活受体α]

[PPARA;PPARA;PPAR;NR1C1;hPPAR;PPARalpha;NR1C1;PPAR;过氧化物酶体增殖物激活受体α]

PPARA_HUMAN

大鼠

468

This kit recognizes Rat PINP in samples. No significant cross-reactivity or interference between Rat PINP and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.94ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat PINP were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat PINP were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat PINP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PINP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PINP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PINP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat PINP. You can calculate the concentration of Rat PINP in the samples by comparing the OD of the samples to the standard curve.

7549811

NP_005027.2

NM_005036.4

Q07869

18,942 Da

Activation Of Gene Expression By SREBF (SREBP) Pathway (685552); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); Developmental Biology Pathway (477129); Energy Metabolism Pathway (198907); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937)

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 22q13.31. Cellular Component: nucleoplasm; nucleus. Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; protein binding; NFAT protein binding; DNA binding; zinc ion binding; ubiquitin conjugating enzyme binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; drug binding; transcription factor activity; lipid binding; phosphatase binding. Biological Process: circadian rhythm; epidermis development; wound healing; positive regulation of transcription, DNA-dependent; heart development; behavioral response to nicotine; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; fatty acid metabolic process; negative regulation of appetite; response to insulin stimulus; positive regulation of gluconeogenesis; circadian regulation of gene expression; negative regulation of blood pressure; negative regulation of protein binding; negative regulation of glycolysis; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; lipoprotein metabolic process; regulation of circadian rhythm; positive regulation of fatty acid beta-oxidation; positive regulation of fatty acid oxidation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; lipid metabolic process; fatty acid transport

24-Strip-Wells

215美元

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590