ELISA/assay

人类紧密连接蛋白-1(紧密连接蛋白ZO-1)酶联免疫吸附试剂盒

MBS2509727

24-Strip-Wells

215美元

酶联免疫吸附试剂盒

人类紧密连接蛋白-1(紧密连接蛋白ZO-1)酶联免疫吸附试剂盒

[紧密连接蛋白-1]

[Tight junction protein ZO-1; Tight junction protein ZO-1; tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein; tight junction protein 1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1]

[紧密连接蛋白-1]

[紧密连接蛋白-1;紧密连接蛋白-1;ZO-1;ZO1]

ZO1_HUMAN

人类

1748

This kit recognizes Human THP in samples. No Significant cross-reactivity or interference between Human THP and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100 ng/mL. Sensitivity: 0.94 ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human THP were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human THP were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human THP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human THP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human THP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human THP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human THP. You can calculate the concentration of Human THP in the samples by comparing the OD of the samples to the standard curve.

85700443

Q07157.3

186,966 Da

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cell Adhesion Proteins Pathway (105680); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); E-cadherin Signaling In The Nascent Adherens Junction Pathway (138005); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (83102); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (515); Gap Junction Pathway (83072)

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014]

ZO1: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with tight junctions (TJ). May mediate dynamic changes in the composition of TJs. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting the protein kinase MRCKb to the leading edge of migrating cells. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Forms homodimers and heterodimers with ZO-2 or ZO-3. Interacts with occludin, CALM, claudins, CGN, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with MRCKb and MYZAP. Interacts (via PDZ domain) with Cx43. ZO-2 and ZO-3 shuttle between the TJ and the nucleus, where they may regulate gene expression. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins can be up- or downregulated in cancer and are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Alternative splicing produces two isoforms. The long isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 15q13. Cellular Component: apical junction complex; intercellular canaliculus; tight junction; basolateral plasma membrane; apicolateral plasma membrane; cytosol; cell-cell adherens junction; apical part of cell; apical plasma membrane; cytoplasm; gap junction; plasma membrane; cell junction; nucleus. Molecular Function: protein C-terminus binding; calmodulin binding; protein domain specific binding; protein binding. Biological Process: response to drug; intercellular junction assembly; response to ethanol; sensory perception of sound; apoptosis; negative regulation of vascular permeability; response to lipopolysaccharide; blastocyst formation; cell structure disassembly during apoptosis

24-Strip-Wells

215美元

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590