产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
Human NEFL (Neurofilament, Light Polypeptide) ELISA Kit
目录 :
MBS2510999
规格 :
24-Strip-Wells
价格 :
215美元
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS2510999 图像 1
产品信息
目录号 :
MBS2510999
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
Human NEFL (Neurofilament, Light Polypeptide) ELISA Kit
产品简称 :
[NEFL]
其他名称 :
[neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; light molecular weight neurofilament protein; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; protein phosphatase 1, regulatory subunit 110; neurofilament, light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein]
产品基因名称 :
[NEFL]
其他基因名称 :
[NEFL;NEFL;NFL;NF-L;NF68;CMT1F;CMT2E;PPP1R110;NF68;NFL;NF-L]
UniProt数据库进入名 :
NFL_HUMAN
反应物种 :
人类
序列长度 :
543
特异性 :
This kit recognizes Human NE/ELA2 in samples. No significant cross-reactivity or interference between HumanNE/ELA2and analogues was observed.
储存稳定性 :
Store at 4 degree C.
image1头 :
Typical Testing Data/Standard Curve (for reference only)
其它信息1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.78-50ng/mL. Sensitivity: 0.47ng/mL.
其他信息2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level HumanNE/ELA2 were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanNE/ELA2 were testedon 3 different plates, 20 replicates in eachplate.
产品描述 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human NE/ELA2 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NE/ELA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NE/ELA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are addedsuccessively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NE/ELA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NE/ELA2. You can calculate the concentration of Human NE/ELA2 in the samples by comparing the OD of the samples to the standard curve.
NCBI GI登录号 :
105990539
NCBI登录号 :
NP_006149.2
NCBI基因登录号 :
NM_006158.4
UniProt数据库登录号 :
P07196
NCBI分子量 :
61,517 Da
NCBI信号通路 :
Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Post NMDA Receptor Activation Events Pathway (161035); Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway (161037)
NCBI总结 :
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
UniProt数据库总结 :
NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 8p21. Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol. Molecular Function: protein binding, bridging; protein C-terminus binding; protein domain specific binding; identical protein binding; protein binding; phospholipase binding; structural constituent of cytoskeleton. Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; axon regeneration in the peripheral nervous system; retrograde axon cargo transport; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; locomotion; neuromuscular process controlling balance; anterograde axon cargo transport; neurite morphogenesis; response to corticosterone stimulus. Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f
尺寸1 :
24-Strip-Wells
价格1 :
215美元
尺寸2 :
48-Strip-Wells
价格2 :
410
size3 :
96-Strip-Wells
价格3 :
490
size4 :
5x96-Strip-Wells
price4 :
2040
size5 :
10x96-Strip-Wells
price5 :
3590
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。