ELISA/assay

大鼠GLUT2(葡萄糖转运蛋白2)酶联免疫吸附试剂盒

MBS2512985

24-Strip-Wells

215美元

酶联免疫吸附试剂盒

大鼠GLUT2(葡萄糖转运蛋白2)酶联免疫吸附试剂盒

[GLUT2]

[GLUT2, partial; Solute carrier family 2, facilitated glucose transporter member 2; solute carrier family 2, facilitated glucose transporter member 2; glucose transporter type 2, liver; liver-type glucose transporter; solute carrier family 2 (facilitated glucose transporter), member 2; Glucose transporter type 2, liver; GLUT-2]

[Glut2]

[Slc2a2; Slc2a2; Glut2; Glut-2; AI266973; Glut-2; Glut2; GLUT-2]

GTR2_MOUSE

大鼠

5

This kit recognizes Rat GLP-1 in samples. No significant cross-reactivity or interference between Rat GLP-1and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 15.63-1000pg/mL. Sensitivity: 9.38pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level Rat GLP-1were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level Rat GLP-1were testedon 3 different plates, 20replicates in eachplate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat GLP-1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GLP-1. St andards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat GLP-1. You can calculate the concentration of Rat GLP-1 in the samples by comparing the OD of the samples to the standard curve.

683589

CAA55368.1

P14246

57,107 Da

Carbohydrate Digestion And Absorption Pathway (170722); Carbohydrate Digestion And Absorption Pathway (170654); Central Carbon Metabolism In Cancer Pathway (1060315); Central Carbon Metabolism In Cancer Pathway (1084231); Developmental Biology Pathway (1111057); Disease Pathway (1111319); Facilitative Na+-independent Glucose Transporters Pathway (1111287); Glucose Transport Pathway (1110800); Glycogen Storage Diseases Pathway (1111374); Glycolysis And Gluconeogenesis Pathway (198376)

GLUT2: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Present at high levels in the plasma membrane of pancreatic b-cells where it initiates the first step in glucose-stimulated insulin secretion, a glucose metabolism-dependent signaling mechanism. May mediate the bidirectional transfer of glucose across the plasma membrane of hepatocytes. May also participate with the Na( +2)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Required for glucose sensitivity in the hypothalamus and brain stem. Involved in the control of food intake and stimulation of glucose uptake by peripheral tissues. Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass. Cellular Component: membrane; brush border membrane; basolateral plasma membrane; cytoplasm; integral to membrane; plasma membrane; cytosol; endosome; brush border. Molecular Function: D-glucose transmembrane transporter activity; dehydroascorbic acid transporter activity; transporter activity; glucose transmembrane transporter activity; transmembrane transporter activity; substrate-specific transmembrane transporter activity; insulin receptor binding. Biological Process: carbohydrate utilization; transport; carbohydrate transport; glucose transport; transmembrane transport

24-Strip-Wells

215美元

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590