ELISA/assay

小鼠ALB(白蛋白)酶联免疫吸附试剂盒

MBS2516177

24-Strip-Wells

215美元

酶联免疫吸附试剂盒

小鼠ALB(白蛋白)酶联免疫吸附试剂盒

[ALB]

[ALB, partial; Serum albumin; serum albumin; albumin (32 AA); albumin (AA 34); cell growth inhibiting protein 42; growth-inhibiting protein 20; albumin]

[ALB]

[ALB;ALB;FDAH;ANALBA;PRO0883;PRO0903;PRO1341]

ALBU_HUMAN

小鼠

167

This kit recognizes Mouse 4-HNE in samples. No significant cross-reactivity or interference between Mouse 4-HNE and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 1.56-100ng/mL. Sensitivity: 0.94ng/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level Mouse4-HNE were tested 20 times onone plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level Mouse4-HNE were testedon 3 different plates, 20 replicates in eachplate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse 4-HNE concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse 4-HNE. During the reaction, Mouse 4-HNE in the sample or standard competes with a fixed amount of Mouse 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse 4-HNE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

37222202

AAQ89947.1

45,160 Da

Bile Acid And Bile Salt Metabolism Pathway (106144); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); HDL-mediated Lipid Transport Pathway (106158); Hemostasis Pathway (106028); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Platelet Activation, Signaling And Aggregation Pathway (106034)

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Carrier; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus. Molecular Function: antioxidant activity; toxin binding; protein binding; copper ion binding; enzyme binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding. Biological Process: platelet activation; receptor-mediated endocytosis; sodium-independent organic anion transport; maintenance of mitochondrion localization; bile acid metabolic process; lipoprotein metabolic process; hemolysis by symbiont of host red blood cells; cellular response to starvation; response to organic substance; response to mercury ion; bile acid and bile salt transport; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; positive regulation of circadian sleep/wake cycle, non-REM sleep; transmembrane transport; response to nutrient; negative regulation of apoptosis. Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic

24-Strip-Wells

215美元

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590