人类, 小鼠, 大鼠
免疫印迹, 免疫组化, 免疫细胞化学
图像 1 :
Western blot analysis of extracts of various cell lines, using IRF5 antibody.
图像 2 :
Immunofluorescence analysis of HeLa cell using IRF5 antibody. Blue: DAPI for nuclear staining.
[interferon regulatory factor 5 isoform e; Interferon regulatory factor 5; interferon regulatory factor 5; interferon regulatory factor 5]
人类, 小鼠, 大鼠
Store at -20 degree C, Avoid freeze / thaw cycles
免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫荧光(IF)
WB: 1:500-1:2000, IHC: 1:50-1:200, IF :1:50-1:200
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-98651
Background/Introduction: Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3gamma/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).IRF-5 is expressed in lymphoid tissues and peripheral blood lymphocytes and participates in the induction of type I interferon genes following viral infection (3). Activation of IRF-5 signaling is triggered by the toll-like receptor (TLR) pathway, including TLR7 and MyD88 (4,5). Genetic variants of IRF-5 have been associated with disorders where the IFN pathway is abnormally activated, such as systemic lupus erythematosus (6,7).
NCBI GI登录号 :
Apoptosis Pathway (198797); Cytokine Signaling In Immune System Pathway (366171); Direct P53 Effectors Pathway (137939); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104); Interferon Gamma Signaling Pathway (187106); Regulation Of Toll-like Receptor Signaling Pathway (920955); Senescence And Autophagy Pathway (198780); Toll-like Receptor Signaling Pathway (83076)
This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010]
IRF5: Transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. Activated by TLR7 or TLR8 signaling. Homodimer, when phosphorylated. Belongs to the IRF family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 7q32. Cellular Component: cytoplasm; cytosol; nucleus. Molecular Function: protein binding; transcription factor activity. Biological Process: transcription, DNA-dependent; positive regulation of interleukin-12 production; positive regulation of apoptosis; response to muramyl dipeptide; cytokine and chemokine mediated signaling pathway; response to peptidoglycan; positive regulation of interferon-beta production; positive regulation of transcription from RNA polymerase II promoter; defense response to virus; positive regulation of interferon-alpha production. Disease: Systemic Lupus Erythematosus, Susceptibility To, 10; Inflammatory Bowel Disease 14
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