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[DYT14, DYT5b, TYH]
[tyrosine 3-monooxygenase isoform b; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; tyrosine hydroxylase; Tyrosine 3-hydroxylase; TH]
> 94 % as determined by SDS-PAGE
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol, 5% trehalose, 5% mannitol, 0.01%Tween80
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -70°C.
Expression Host: Baculovirus-Insect Cells. Predicted N Terminal: Met
Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method. Reconstitution: It is recommened that sterile water (80 ul) be added to the vial to prepare stock solution of 0.25 mg/ml. Concentration is measured by UV-Vis.
Background: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA. Description: A DNA sequence encoding the human TH isoform 2 (P07101-3) (Pro 2-Gly 497) was fused with a polyhistidine tag at the N-terminus.
NCBI GI登录号 :
The recombinant human TH consists of 515 amino acids and has a calculated molecular mass of 57.6 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
ATF-2 Transcription Factor Network Pathway (138006); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Alpha-synuclein Signaling Pathway (137913); Amine-derived Hormones Pathway (160983); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198793); Catecholamine Biosynthesis Pathway (160984); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357)
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described. Protein type: Amino Acid Metabolism - tyrosine; EC 18.104.22.168; Endoplasmic reticulum; Mitochondrial; Oxidoreductase; Vesicle. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; internal side of plasma membrane; melanosome membrane; neuron projection; nucleus; perikaryon; smooth endoplasmic reticulum. Molecular Function: enzyme binding; protein binding; tyrosine 3-monooxygenase activity. Biological Process: anatomical structure morphogenesis; catecholamine biosynthetic process; dopamine biosynthetic process; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; eye photoreceptor cell development; heart development; learning; locomotory behavior; memory; norepinephrine biosynthetic process; organ morphogenesis; pigmentation; regulation of heart contraction; response to ethanol; response to hypoxia; synaptic transmission, dopaminergic; visual perception. Disease: Segawa Syndrome, Autosomal Recessive
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San Diego, CA 92195-3308