产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
豚鼠白细胞介素10(IL-10)酶联免疫吸附试剂盒
目录 :
MBS262431
规格 :
48-Strip-Wells
价格 :
315美元
更多信息或购买 :
产品信息
目录号 :
MBS262431
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
豚鼠白细胞介素10(IL-10)酶联免疫吸附试剂盒
产品简称 :
白细胞介素10(IL-10)
其他名称 :
interleukin-10; Interleukin-10; interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor; interleukin 10; Cytokine synthesis inhibitory factor; CSIF
产品基因名称 :
IL-10
其他基因名称 :
白细胞介素10;白细胞介素10;CSIF;TGIF;GVHDS;IL-10;IL10A;IL-10;CSIF
UniProt数据库进入名 :
IL10_HUMAN
反应物种 :
豚鼠
序列长度 :
178
特异性 :
No cross-reaction with other factors.
储存稳定性 :
Store all reagents at 2-8 degree C.
其它信息1 :
Samples: Guinea pig serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant IL-10 concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
其他信息2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
产品描述 :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Guinea pig IL-10 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
NCBI GI登录号 :
10835141
NCBI登录号 :
NP_000563.1
NCBI基因登录号 :
NM_000572.2
NCBI分子量 :
20,517 Da
NCBI信号通路 :
African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)
NCBI总结 :
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
UniProt数据库总结 :
IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family. Protein type: Secreted; Motility/polarity/chemotaxis; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q31-q32. Cellular Component: extracellular space. Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding. Biological Process: B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; regulation of isotype switching; positive regulation of transcription, DNA-dependent; leukocyte chemotaxis; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; negative regulation of T cell proliferation; cytoplasmic sequestering of NF-kappaB; cell-cell signaling; response to molecule of bacterial origin; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor biosynthetic process; hemopoiesis; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; T-helper 2 type immune response; positive regulation of B cell apoptosis; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to activity; receptor biosynthetic process; regulation of sensory perception of pain; positive regulation of cytokine secretion; negative regulation of apoptosis. Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To
尺寸1 :
48-Strip-Wells
价格1 :
315美元
尺寸2 :
96-Strip-Wells
价格2 :
490
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。