ELISA/assay

Rat Leukemia Inhibitory Factor (LIF) ELISA kit

MBS263914

48-Strip-Wells

290美元

酶联免疫吸附试剂盒

Rat Leukemia Inhibitory Factor (LIF) ELISA kit

Leukemia Inhibitory Factor (LIF)

leukemia inhibitory factor isoform 1; Leukemia inhibitory factor; leukemia inhibitory factor; D factor; human interleukin in DA cells; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; differentiation-stimulating factor; differentiation inhibitory activity; leukemia inhibitory factor; Differentiation-stimulating factor; D factor; Melanoma-derived LPL inhibitor; MLPLI; INN: Emfilermin

LIF

LIF; LIF; CDF; DIA; HILDA; MLPLI; HILDA; LIF; D factor; MLPLI

LIF_HUMAN

大鼠

202

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant LIF concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat LIF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4504991

NP_002300.1

NM_002309.4

9,563 Da

Adipogenesis Pathway (198832); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Direct P53 Effectors Pathway (137939); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); TGF Beta Signaling Pathway (198810); TNF Signaling Pathway (812256); TNF Signaling Pathway (813210)

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

LIF: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. Belongs to the LIF/OSM family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 22q12.2. Cellular Component: extracellular space; cytoplasm. Molecular Function: growth factor activity; leukemia inhibitory factor receptor binding; cytokine activity; receptor binding. Biological Process: transcription from RNA polymerase II promoter; leukemia inhibitory factor signaling pathway; negative regulation of hormone secretion; multicellular organismal development; stem cell maintenance; tyrosine phosphorylation of Stat3 protein; decidualization; positive regulation of peptidyl-serine phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of cell proliferation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; negative regulation of meiosis; positive regulation of cell proliferation; neuron development; immune response; positive regulation of transcription from RNA polymerase II promoter; blood vessel remodeling; positive regulation of macrophage differentiation; muscle morphogenesis; alveolus development; embryo implantation; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of astrocyte differentiation

48-Strip-Wells

290美元

96-Strip-Wells

450