ELISA/assay

大鼠胱氨酸-天冬氨酸蛋白酶9(胱氨酸-天冬氨酸蛋白酶9)酶联免疫吸附试剂盒

MBS264306

48-Strip-Wells

290美元

酶联免疫吸附试剂盒

大鼠胱氨酸-天冬氨酸蛋白酶9(胱氨酸-天冬氨酸蛋白酶9)酶联免疫吸附试剂盒

胱氨酸-天冬氨酸蛋白酶9(胱氨酸-天冬氨酸蛋白酶9)

caspase-9; Caspase-9; caspase-9; apoptotic protease MCH-6; ICE-like apoptotic protease 6; apoptotic protease activating factor 3; protein phosphatase 1, regulatory subunit 56; caspase 9, apoptosis-related cysteine peptidase; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

CASP9;CASP9;MCH6;APAF3;APAF-3;PPP1R56;ICE-LAP6;MCH6;CASP-9;APAF-3;ICE-LAP6

CASP9_HUMAN

大鼠

266

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Caspase-9 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat Caspase-9 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

6518502

BAA87905.1

36,564 Da

AGE/RAGE Pathway (698754); AKT Phosphorylates Targets In The Cytosol Pathway (106475); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Adaptive Immune System Pathway (366160); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797)

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

CASP9: a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two isoforms. Protein type: EC 3.4.22.62; Apoptosis; Protease. Chromosomal Location of Human Ortholog: 1p36.21. Cellular Component: mitochondrion; cytosol; nucleus; apoptosome. Molecular Function: peptidase activity; protein binding; cysteine-type endopeptidase activity; enzyme activator activity; SH3 domain binding; protein kinase binding. Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; DNA damage response, signal transduction; response to lipopolysaccharide; proteolysis; response to estradiol stimulus; response to antibiotic; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of neuron apoptosis; response to cobalt ion; innate immune response; platelet formation; caspase activation via cytochrome c; response to DNA damage stimulus; aging

48-Strip-Wells

290美元

96-Strip-Wells

450