ELISA/assay

大鼠孕酮(PROG)酶联免疫吸附试剂盒

MBS265343

48-Strip-Wells

290美元

酶联免疫吸附试剂盒

大鼠孕酮(PROG)酶联免疫吸附试剂盒

孕酮(PROG)

progesterone receptor isoform B; Progesterone receptor; progesterone receptor; nuclear receptor subfamily 3 group C member 3; progesterone receptor; Nuclear receptor subfamily 3 group C member 3

PROG

PGR;PGR;PR;NR3C3;NR3C3;PR

PRGR_HUMAN

大鼠

933

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PROG concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

. Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PROG monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

110611914

NP_000917.3

NM_000926.4

87,747 Da

Cellular Roles Of Anthrax Toxin Pathway (138076); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); Nuclear Signaling By ERBB4 Pathway (530744); Oocyte Meiosis Pathway (126909); Oocyte Meiosis Pathway (126872); Ovarian Infertility Genes Pathway (198801); Progesterone-mediated Oocyte Maturation Pathway (119304)

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011]

PR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 11q22-q23. Cellular Component: nucleoplasm; mitochondrial outer membrane. Molecular Function: ligand-dependent nuclear receptor activity; protein binding; enzyme binding; DNA binding; zinc ion binding; steroid hormone receptor activity; steroid binding; receptor binding. Biological Process: transcription initiation from RNA polymerase II promoter; progesterone receptor signaling pathway; cell-cell signaling; epithelial cell maturation; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; ovulation from ovarian follicle; signal transduction; regulation of epithelial cell proliferation. Disease: Progesterone Resistance

48-Strip-Wells

290美元

96-Strip-Wells

450