产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
人类血红素氧化酶(HO-1)酶联免疫吸附试剂盒
目录 :
MBS268886
规格 :
48-Strip-Wells
价格 :
275美元
更多信息或购买 :
产品信息
目录号 :
MBS268886
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
人类血红素氧化酶(HO-1)酶联免疫吸附试剂盒
产品简称 :
血红素氧化酶(HO-1)
其他名称 :
heme oxygenase 1; Heme oxygenase 1; heme oxygenase 1; heat shock protein, 32-kD; heme oxygenase (decycling) 1
产品基因名称 :
HO-1
其他基因名称 :
HMOX1;HMOX1;HO-1;HSP32;HMOX1D;bK286B10;HO;HO1;HO-1
UniProt数据库进入名 :
HMOX1_HUMAN
反应物种 :
人类
序列长度 :
288
特异性 :
No cross-reaction with other factors.
储存稳定性 :
Store all reagents at 2-8 degree C.
其它信息1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HO-1 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
其他信息2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
产品描述 :
. Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HO-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
NCBI GI登录号 :
4504437
NCBI登录号 :
NP_002124.1
NCBI基因登录号 :
NM_002133.2
NCBI分子量 :
32,819 Da
NCBI信号通路 :
HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Heme Degradation Pathway (160971); Iron Uptake And Transport Pathway (187191); Keap1-Nrf2 Pathway (198777); Metabolism Pathway (477135); Metabolism Of Porphyrins Pathway (106325); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Oxidative Stress Pathway (198916)
NCBI总结 :
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family. Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3; Oxidoreductase. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; nucleolus; caveola; nucleus; cytosol. Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; metal ion binding; phospholipase D activity; heme binding; heme oxygenase (decyclizing) activity. Biological Process: cell death; response to nicotine; negative regulation of smooth muscle cell proliferation; cellular iron ion homeostasis; positive regulation of smooth muscle cell proliferation; negative regulation of mast cell degranulation; positive regulation of vasodilation; excretion; erythrocyte homeostasis; regulation of transcription factor activity; heme catabolic process; regulation of blood pressure; small GTPase mediated signal transduction; porphyrin metabolic process; negative regulation of mast cell cytokine production; negative regulation of neuron apoptosis; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; healing during inflammatory response; transmembrane transport; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; regulation of angiogenesis; negative regulation of leukocyte migration; negative regulation of DNA binding; positive regulation of angiogenesis; iron ion homeostasis; positive regulation of chemokine biosynthetic process; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to estrogen stimulus; endothelial cell proliferation; response to oxidative stress; smooth muscle hyperplasia. Disease: Heme Oxygenase 1 Deficiency; Pulmonary Disease, Chronic Obstructive
尺寸1 :
48-Strip-Wells
价格1 :
275美元
尺寸2 :
96-Strip-Wells
价格2 :
420
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。