ELISA/assay

Pig Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

MBS2700218

24-Strip-Wells

230美元

酶联免疫吸附试剂盒

Pig Fibroblast Growth Factor 2, Basic (FGF2) ELISA Kit

[Fibroblast Growth Factor 2, Basic (FGF2)]

[B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2]

[fibroblast growth factor 2 34 kDa isoform; Fibroblast growth factor 2; fibroblast growth factor 2; fibroblast growth factor 2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2]

[纤维生长因子2]

[纤维生长因子2;纤维生长因子2;BFGF;FGFB;FGF-2;HBGF-2;FGFB;FGF-2;bFGF;HBGF-2]

猪

288

This assay has high sensitivity and excellent specificity for detection of FGF2. No significant cross-reactivity or interference between FGF2 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 12.35-1,000pg/mL. Sensitivity: 5.16pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FGF2 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to FGF2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FGF2 and unlabeled FGF2 (Standards or samples) with the pre-coated antibody specific to FGF2. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FGF2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FGF2 in the sample.

153285461

NP_001997.5

NM_002006.4

P09038

21,203 Da

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cardiac Progenitor Differentiation Pathway (712094); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Developmental Biology Pathway (477129); Disease Pathway (530764)

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).

24-Strip-Wells

230美元

48-Strip-Wells

380

96-Strip-Wells

505

5x96-Strip-Wells

2115

10x96-Strip-Wells

3930