蛋白

重组人类MMP1蛋白, 重组人类基质金属蛋白酶1(MMP1)蛋白

MBS286117

0.1毫克

370美元

重组蛋白

重组人类MMP1蛋白, 重组人类基质金属蛋白酶1(MMP1)蛋白

[MMP1]

[CLGN;CLG1;胶原酶;间质胶原酶;脊椎动物胶原酶;成纤维细胞胶原酶]

[interstitial collagenase isoform 1 preproprotein; Interstitial collagenase; interstitial collagenase; matrix metallopeptidase 1; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1]

[MMP1]

[MMP1;MMP1;CLG;CLGN;CLG;基质金属蛋白酶-1]

MMP1_HUMAN

大肠杆菌氨基酸101-469(P03956)

[101-469]

469

VLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQ
LWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPG
GNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAA
HELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGI
QAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVM
FFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEF
ADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRT
VKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYP
KMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKT
KRILTLQKANSWFNCRKN

> 90 % as determined by SDS-PAGE.

在10 mM三羟甲基氨基甲烷氯化氢, 1 mM乙二胺四乙酸, pH值8.0, 50% 甘油

0.8毫克/毫升

Store it under sterile conditions at -20°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. **Avoid repeated freeze-thaw cycles.**. Stability: The recombinant protein is stable for up to 6 months from date of receipt at -80°C.

免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA)

十二烷基磺酸钠-PAGE

Source: Human. Predicted Molecular Mass: Predicted MW: 46.5 kDa. Observed MW: 46.5 kDa

Protein Residues: with N-terminal 6×His-tag. Usage: MMP1 Protein - Centrifuge the standard vial at 6000-10000rpm for 30s.

MMP-1 is produced by fibroblasts, chondrocytes, macrophages, endothelial cells, and osteoblasts. It is induced by the pro-inflammatory cytokines IL-1 and TNF-alpha, various growth factors such as EGF, PDGF, FGF basic, and Oncostatin M, chemical agents such as cAMP and phorbol esters and events occurring at the cell surface such as cell fusion and phagocytosis. MMP-1 plays a significant role in the degradation of different types of collagen in extracellular matrix remodeling. It is implicated in a variety of processes involving collagen degradation such as emphysema, atherosclerosis, rheumatoid and osteoarthritis, periodontal and respiratory disease, angiogenesis and tumorigenesis, tissue remodeling and wound healing, and inflammatory bowel disease.

4505215

NP_002412.1

NM_002421.3

P03956

Activation Of Matrix Metalloproteinases Pathway (1270258); Basigin Interactions Pathway (1269376); Bladder Cancer Pathway (1458294); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Collagen Degradation Pathway (1270259); Cytokine Signaling In Immune System Pathway (1269310); Degradation Of The Extracellular Matrix Pathway (1270257); Endothelins Pathway (137958)

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

MMP1: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family. Protein type: EC 3.4.24.7; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q22.3. Cellular Component: extracellular region. Molecular Function: endopeptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity. Biological Process: cellular protein metabolic process; collagen catabolic process; extracellular matrix disassembly; leukocyte migration; positive regulation of protein oligomerization; proteolysis. Disease: Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Pulmonary Disease, Chronic Obstructive

0.1毫克

370美元

0.5毫克

1045

1毫克

1455