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Recombinant and natural Rat Protein S100-B
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI GI登录号 :
Activated TLR4 Signalling Pathway (1332818); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1332837); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1332845); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1332853); Immune System Pathway (1332754); Innate Immune System Pathway (1332786); MyD88 Cascade Initiated On Plasma Membrane Pathway (1332790); MyD88 Dependent Cascade Initiated On Endosome Pathway (1332810); MyD88-independent TLR3/TLR4 Cascade Pathway (1332804); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (1332819)
binds GTPase activating protein IQGAP1; may play a role in cell membrane rearrangement [RGD, Feb 2006]
S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. Protein type: Calcium-binding. Cellular Component: cell soma; cytoplasm; extracellular space; intracellular membrane-bound organelle; nucleus; perinuclear region of cytoplasm; ruffle. Molecular Function: calcium ion binding; calcium-dependent protein binding; identical protein binding; protein binding; protein homodimerization activity; RAGE receptor binding; receptor binding; tau protein binding; zinc ion binding. Biological Process: astrocyte differentiation; learning and/or memory; memory; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of synaptic transmission; regulation of cell shape; regulation of neuronal synaptic plasticity; response to glucocorticoid stimulus; response to methylmercury
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San Diego, CA 92195-3308