ELISA/assay

Human Hyaluronan synthase 1 ELISA Kit

MBS289944

48-Strip-Wells

400美元

酶联免疫吸附试剂盒

Human Hyaluronan synthase 1 ELISA Kit

[Hyaluronan synthase 1]

[Hyaluronan synthase 1; 2.4.1.212; Hyaluronate synthase 1; Hyaluronic acid synthase 1; HA synthase 1; HAS1; HAS]

[hyaluronan synthase 1 isoform 1; Hyaluronan synthase 1; hyaluronan synthase 1; hyaluronan synthase 1; Hyaluronate synthase 1; Hyaluronic acid synthase 1; HA synthase 1; HuHAS1]

[HAS1]

[HAS1; HAS1; HAS; HAS; HA synthase 1; HuHAS1]

HYAS1_HUMAN

人类

578

For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Assay Type: Sandwich. Detection Range: 0.156-10 ng/mL. Sensitivity: 0.098ng/mL

Intra-Assay CV: <=4.9%. Inter-Assay CV: <=9.5%. Recovery: 107%

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

167466171

NP_001514.2

NM_001523.3

Q92839

64,832 Da

Glycosaminoglycan Metabolism Pathway (1269972); Hyaluronan Biosynthesis And Export Pathway (1269974); Hyaluronan Metabolism Pathway (1269973); Metabolism Pathway (1269956); Metabolism Of Carbohydrates Pathway (1269957)

Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS1 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase (DG42) from Xenopus laevis, and a recently described murine hyaluronan synthase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

HAS1: Plays a role in hyaluronan/hyaluronic acid (HA) synthesis. Also able to catalyze the synthesis of chito- oligosaccharide depending on the substrate. Belongs to the NodC/HAS family. Protein type: Cell adhesion; EC 2.4.1.212; Motility/polarity/chemotaxis; Membrane protein, integral; Membrane protein, multi-pass; Transferase. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: cytoplasm; integral to plasma membrane; plasma membrane. Molecular Function: hyaluronan synthase activity. Biological Process: carbohydrate metabolic process; cell adhesion; extracellular polysaccharide biosynthetic process; glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; hyaluronan biosynthetic process; hyaluronan metabolic process

48-Strip-Wells

400美元

96-Strip-Wells

530

5x96-Strip-Wells

2305

10x96-Strip-Wells

4250