抗体

兔抗人类ALK/p80单克隆抗体(克隆SP8)

MBS302571

0.1毫升

265美元

单克隆

兔

nonconjugated

SP8

人类, 小鼠

免疫组化, 免疫组化-石蜡切片

抗体

兔抗人类ALK/p80单克隆抗体(克隆SP8)

ALK/p80

ALK tyrosine kinase receptor; ALK tyrosine kinase receptor; ALK tyrosine kinase receptor; CD246 antigen; mutant anaplastic lymphoma kinase; anaplastic lymphoma receptor tyrosine kinase; Anaplastic lymphoma kinase; CD_antigen: CD246

ALK/p80

ALK;ALK;CD246;NBLST3

ALK_HUMAN

单克隆

IgG

SP8

兔

Human. Predicted Reactivity: Mouse

1620

0.1 ml rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

免疫组化(免疫组化)石蜡

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Anaplastic Lymphoma

Immunogen: Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript. Control Tissue: Anaplastic Lymphoma. Cellular Localization: Cytoplasm, Nucleus. Entrez Gene Code: 238. Pathway: Oncogenesis

This antibody recognizes a human p80 protein, identified as a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in a third of large cell lymphomas. This antibody can be used to detect p80 in these lymphomas and may also be used to detect a subtype of large B cell lymphoma, which expresses the full-length ALK protein.

29029632

NP_004295.2

NM_004304.4

80 kDa

Non-small Cell Lung Cancer Pathway 83119!!Non-small Cell Lung Cancer Pathway 531

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

0.1毫升

265美元