抗体

MAb to glyceraldehyde-3-PDH

MBS311075

1毫克

490美元

单克隆

小鼠

未共轭

4G5

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

MAb to glyceraldehyde-3-PDH

甘油醛3脱氢酶(磷酸甘油醛脱氢酶)

单克隆抗体甘油醛3脱氢酶(磷酸甘油醛脱氢酶)

glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; OTTHUMP00000174430; OTTHUMP00000174431; OTTHUMP00000174432; OTTHUMP00000174434; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH

磷酸甘油醛脱氢酶;磷酸甘油醛脱氢酶;G3PD;GAPD;MGC88685;GAPD

G3P_HUMAN

单克隆

IgG1

4G5

Host: Mouse. Hybridization of Sp2/0 myeloma. cells with spleen cells from BALB/c mice. Source: Ascites

兔

335

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Recognizes GAPDH monomer (36 kDa) and the dimer form. Does not react with the tetrameric form. Reacts with GAPDH from human, bovine, pig, goat, dog, rabbit, cat, rat, mouse and fish.

>90% pure (SDS-PAGE). Protein A chromatography

纯化, 液体

4.4mg/ml (Sigma protein assay kit)

Store at 2 to 8 degree C.

EIA/酶联免疫吸附测定, 免疫测定抗体配对, 免疫印迹

Immunogen: Rabbit muscle GAPDH. Affinity Constant: Not determined

Buffer: PBS, pH 7.4. Preservative: 0.1% Sodium azide. Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Monoclonal Antibodies to Enzymes

FEBS Letters, (1992), Volume 300, number 1, 9-12.

7669492

NP_002037.2

NM_002046.3

P04406

36,053 Da

Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Androgen Receptor Signaling Pathway 198806!!Gluconeogenesis Pathway 106204!!Glucose Metabolism Pathway 106199!!Glycolysis Pathway 105911!!Glycolysis / Gluconeogenesis Pathway 82926!!Glycolysis / Gluconeogenesis Pathway 287!!Metabolic Pathways 132956!!Metabolism Of Carbohydrates Pathway 106196

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Ref.6 Ref.23. Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Ref.6. Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Subunit structure: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. By similarity. Interacts with EIF1AD, USP25, PRKCI and WARS. Ref.23 Ref.25 Ref.28 Ref.36 Ref.39 Ref.40. Subcellular location: Cytoplasm cytosol. Nucleus. By similarity. Cytoplasm perinuclear region. Membrane. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. By similarity. Postnuclear and Perinuclear regions. Ref.24. Post-translational modification: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. By similarity.ISGylated. Probable. Ref.29. Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

1毫克

490美元