抗体

MAb to CD35

MBS311979

0.2毫克

505美元

单克隆

小鼠

未共轭

J3D3

酶联免疫吸附测定, 中和反应, 流式细胞仪, 酶免疫法

抗体

MAb to CD35

CD35

单克隆抗体CD35, C3b受体160, 190, 220, 和250kDa

单克隆

IgG1, kappa

J3D3

腹水

人类

CD35. CD35 antigen is composed of 4 allotypes. C3b Receptor (CR1) is present on B lymphocytes, monocytes, myeloid cells, dendritic cells of lymphocyte follicules and renal podocytes.

Purified, Lyophilized. Reconstitute with 1ml distilled water.

0.2 mg/ml (prior to lyophilization)

Store lyophilized product at 2 to 8 degree C. After reconstitution, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles. The addition of 0.1% sodium azide is recommended for storage up to one month at 2 to 8 degree C.

Block, EIA/ELISA, Flow Cytometry, Immunohistochemistry - Formalin/Paraffin. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necess

Mechanism of degradation of C3b. Analysis of lymphoid and myeloid differentiation. J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3b dependent rosette formation with lymphocytes. Fluorescence microscopy or flow cytometry: 2ug/5x10^5 cells/tests. Immunohistochemistry: Working dilution: 1:25 to 1:50. Suitable on frozen sections or cell smears and on Bouin's fixed, paraffin-embedded tissues.

Volume: 1ml. Immunogen: Purified C3b receptor from human red blood cells. Affinity Constant: Not determined

Buffer: Lyophilized from PBS containing 1mg/ml BSA. Preservative: No. Lyophilized: Yes. Important Note: Centrifuge before opening to ensure complete recovery of vial contents.

Monoclonal Antibodies to Human CDs

Cook J., et al., (1985), "Mouse monoclonal antibodies to the human C3b receptor", Molec. Immunol., 22 #5, 531-539. Reid, K.B.M., "Complement system protein which interact with C3b or C4d", Immunology Today, 7, 7

0.2毫克

505美元