抗体

羊抗大鼠肾素

MBS315812

1毫克

575美元

多克隆

羊

未共轭

人类, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法

抗体

羊抗大鼠肾素

肾素

羊抗体大鼠肾素

renin; Renin; renin; OTTHUMP00000034311; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme; renin; Angiotensinogenase

REN;REN;HNFJ2;FLJ10761

RENI_HUMAN

多克隆

羊

大鼠

406

肾素。 反应对象大鼠和小鼠肾素

蛋白质G色谱

纯化, 液体

8.7毫克/毫升(OD280nm, E^0.1% = 1.36)

Upon receipt, store at -70 degree C. Avoid multiple freeze/thaw cycles. Shipping: ships on cold packs.

EIA/酶联免疫吸附测定, 免疫印迹, 免疫组化

适合用途包括酶联免疫吸附测定(1:50, 000), 免疫印迹(1:5, 000)和免疫组化(1:1, 000)。 针对某一特定用途每个实验室都应该确定一个最佳的工作滴度。 其他的应用还没有被检测，但用在该实验中应用不应该被排除

Immunogen: Recombinant Rat Prorenin

Buffer: 0.05M Sodium phosphate, 0.1M Sodium chloride, 1mM EDTA, pH 6.6. Preservative: No. Important Note: Centrifuge before opening to ensure complete recovery of vial contents.

Polyclonal Antibodies to Enzymes

Carilli, C.T., et al., (1988), "Semi-preparative purification of recombinant human renin and prorenin", J. Chromatogr., 444: 203-208. Sielecki, A.R., et al., (1989), "Structure of recombinant human renin, a target for cardiovasc

190994

AAA60363.1

P00797

45,057 Da

ACE Inhibitor Pathway (198763); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq]

Function: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Catalytic activity: Cleavage of Leu- -Xaa bond in angiotensinogen to generate angiotensin I. Enzyme regulation: Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Subunit structure: Interacts with ATP6AP2. Ref.14. Subcellular location: Secreted. Membrane. Note: Associated to membranes via binding to ATP6AP2. Involvement in disease: Defects in REN are a cause of renal tubular dysgenesis (RTD) [. MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Ref.17Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [. MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. Ref.18. Sequence similarities: Belongs to the peptidase A1 family. Biophysicochemical propertiesKinetic parameters:KM=1 uM for angiotensinogen (in absence of ATP6AP2)KM=0.15 uM for angiotensinogen (in presence of membrane-bound ATP6AP2)

1毫克

575美元