产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
TGFbeta1小鼠酶联免疫吸附试剂盒
目录 :
MBS355229
规格 :
96试验
价格 :
385美元
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS355229 图像 1
产品信息
目录号 :
MBS355229
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
TGFbeta1小鼠酶联免疫吸附试剂盒
产品简称 :
[TGFbeta1]
产品名称同义词 :
[小鼠TGFbeta1]
产品基因名称 :
[TGFbeta1]
反应物种 :
小鼠
储存稳定性 :
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
检测过的应用 :
定量sELISA(EIA)
应用笔记 :
For quantitative detection of TGFbeta1 in mouse serum, body fluids, tissue lysates or cell culture supernatants.
image1头 :
Typical Testing Data/Standard Curve (for reference only)
其它信息1 :
Samples: mouse serum, body fluids, tissue lysates or cell culture supernatants. Assay Type: Quantitative Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: < 2 pg/ml
产品描述 :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TGFbeta1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TGFbeta1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGFbeta1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGFbeta1 can be calculated. Background: Transforming growth factor beta 1 or TGFbeta1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. In humans, TGFbeta1 is encoded by the TGFB1 gene. This gene contains 7 exons and very large introns, maps to 19q13.1-q13.3. TGFbeta1 acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. The TGFB1 is directly involved in the pathogenesis of bone marrow reticulin fibrosis in hairy cell leukemia. The expression of TGFB1 in the early stages of DMD may be critical in initiating muscle fibrosis, and suggested that antifibrosis treatment might slow progression of the disease, increasing the utility of gene therapy.
尺寸1 :
96试验
价格1 :
385美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。