ELISA/assay

IL-10大鼠酶联免疫吸附试剂盒

MBS355232

96试验

385美元

酶联免疫吸附试剂盒

IL-10大鼠酶联免疫吸附试剂盒

[IL-10]

[大鼠IL-10]

[IL-10;IL-10]

[IL-10]

[SPPV_03]

大鼠

This assay has high sensitivity and excellent specificity for detection of humanin. No significant cross-reactivity or interference between humanin and analogues was observed.

Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

定量sELISA(EIA)

For quantitative detection of IL-10 in rat serum, body fluids, tissue lysates or cell culture supernatants.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level humanin were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level humanin were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Antihumanin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-humanin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the humanin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of humanin can be calculated.

21492460

NP_659579.1

NC_004002.1

96试验

385美元