抗体

bcl-2

MBS370007

5 + Control Slides

115美元

单克隆

小鼠

未共轭

BCL2/A4

人类, 大鼠

抗体

bcl-2

bcl-2

BCL-2; Apoptosis regulator Bcl-2; apoptosis regulator Bcl-2; Bcl2-like protein; B-cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2

bcl-2

Bcl2; Bcl2; Bcl-2; Bcl-2

BCL2_RAT

单克隆

IgG1/K

BCL2/A4

小鼠

人类

Anti-bcl-2 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Store at 2 to 8 degree C in the dark.

Control: Tonsil, Lymph Node. Localization: Cytoplasmic

Reactivity Note: Paraffin, Frozen

bcl-2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of bcl-2, such as in the case of translocation of bcl-2 to Ig heavy-chain loci, is thought to be the cause of Follicular Lymphoma. Anti-bcl-2 has shown consistent negative reaction on reactive germinal centers and positive staining of neoplastic follicles in Follicular Lymphoma. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. This antibody may also be used in distinguishing between those Follicular Lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2-negative. Anti-bcl-2 has been used as a predictive biomarker for recurrence of Cancer of the Breast and Non-Small-Cell Carcinoma of the Lung.

1004379

AAA77687.1

U34964.1

P49950

26,622 Da

ATM Pathway (198524); Activation Of BAD And Translocation To Mitochondria Pathway (368632); Activation Of BH3-only Proteins Pathway (368631); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (219785); Apoptosis Pathway (83452); Apoptosis Pathway (470); Apoptosis Pathway (368621); B Cell Receptor Signaling Pathway (198486)

an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD]

Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Subunit structure: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity. Also interacts with APAF1, BBC3, BCL2L1, BNIPL, EI24, FKBP8, MRPL41, RAF-1, TP53BP2. Interacts with BAG1 in an ATP-dependent manner. Subcellular location: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Tissue specificity: Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, and in ependymal cells. In prenatal brain, expression is higher and localized in the neuroepithelium and in the cortical plate. Domain: The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1. Post-translational modification: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A). By similarity.Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. By similarity.Monoubiquitinated by PARK2, leading to increase its stability. Sequence similarities: Belongs to the Bcl-2 family.

5 + Control Slides

115美元

3 ml (Prediluted)

135

7 ml (Prediluted)

150

0.1 mL (Concentrate)

155

15 ml (Prediluted)

215