抗体

山羊抗GFAP抗体

MBS420746

0.1毫克

300美元

多克隆

山羊

未共轭

人类, 小鼠, 大鼠, 狗

免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法

抗体

山羊抗GFAP抗体

GFAP

GFAP;胶质纤维酸性蛋白;FLJ45472;中间丝蛋白类;GFAP抗体;胶质纤维酸性蛋白抗体;FLJ45472抗体;中间丝蛋白类抗体

glial fibrillary acidic protein isoform 1; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein

GFAP

GFAP;GFAP;ALXDRD;GFAP

GFAP_HUMAN

多克隆

山羊

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog

432

DGEVIKESKQEHKD

通过硫酸铵沉淀和用免疫多肽抗原亲和层析从山羊血清中纯化得到的

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

肽酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹), 免疫组化(免疫组化), 酶联免疫吸附测定(EIA)

Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Cerebellum shows specific staining of astrocytes. Recommended concentration, 2-4ug/ml. Frozen sections of Human Hypothalamus shows staining of astroglial cells. Recommended concentration, 0.01-0. 03ug/ml. Both data obtained from a previous batch (different goat). Western Blot: Approx 48kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 49.9kDa according to Mouse NP_034407.2 and Human NP_002046.1). Recommended concentration: 0.01-0.03ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant GFAP captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. Please note this data was obtained using a previous batch (different goat).

Immunogen: Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1. Epitope: C Terminus

Note: GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.

4503979

NP_002046.1

NM_002055.4

49,508 Da

Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neural Crest Differentiation Pathway (672460); Nuclear Signaling By ERBB4 Pathway (1269499); Signal Transduction Pathway (1269379); Signaling By ERBB4 Pathway (1269496); Spinal Cord Injury Pathway (739007)

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: cytoplasm; cytosol; intermediate filament; intermediate filament cytoskeleton; lysosome; membrane; myelin sheath. Molecular Function: glycoprotein binding; integrin binding; kinase binding; protein binding; structural constituent of cytoskeleton. Biological Process: astrocyte development; Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; intermediate filament organization; neurite regeneration; regulation of neurotransmitter uptake; regulation of protein complex assembly; response to wounding. Disease: Alexander Disease

0.1毫克

300美元