抗体

Goat anti-IFT88/Polaris Antibody

MBS420765

0.1毫克

300美元

多克隆

山羊

未共轭

人类, 小鼠, 大鼠, 狗

免疫组化, 免疫细胞化学, 酶免疫法

抗体

Goat anti-IFT88/Polaris Antibody

IFT88/Polaris

IFT88; polaris; TTC10; tetratricopeptide repeat domain 10; HGNC: 20606; D13S1056E; DAF19; IFT88; MGC26259; RP11-172H24.2; TG737; hTg737; TPR repeat protein 10; Probe hTg737 (polycystic kidney disease, autosomal recessive); Tg737 protein; intraflagellar tra; IFT88 antibody; polaris antibody; TTC10 antibody; tetratricopeptide repeat domain 10 antibody; HGNC: 20606 antibody; D13S1056E antibody; DAF19 antibody; IFT88 antibody; MGC26259 antibody; RP11-172H24.2 antibody; TG737 antibody; hTg737 antibody; TPR repeat protein 10 antibody; Probe hTg737 (polycystic kidney disease; autosomal recessive) antibody; Tg737 protein antibody; intraflagellar transport 88 homolog antibody; recessive polycystic kidney disease protein Tg737 homolog antibody; intraflagellar transport 88 homolog (Chlamydomonas) antibody; Polaris; IFT88 / Polaris

intraflagellar transport protein 88 homolog isoform 1; Intraflagellar transport protein 88 homolog; intraflagellar transport protein 88 homolog; intraflagellar transport 88; Recessive polycystic kidney disease protein Tg737 homolog; Tetratricopeptide repeat protein 10; TPR repeat protein 10

IFT88

IFT88; IFT88; DAF19; TG737; TTC10; hTg737; D13S1056E; TG737; TTC10; TPR repeat protein 10

IFT88_HUMAN

多克隆

山羊

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog

833

KKRIDEDDFADEE

通过硫酸铵沉淀和用免疫多肽抗原亲和层析从山羊血清中纯化得到的

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

肽酶联免疫吸附测定(EIA), 免疫组化(免疫组化), 免疫荧光(IF)

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration, 3ug/ml. Western Blot: Preliminary experiments in Human Kidney, Liver and Lung lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). Immunofluorescence: This antibody has been successfully used in IF for mouse nose and lungs as described in the following paper: Miyoshi et al, FASEB J.2009 Oct; 23(10): 3289-97; PMID: 19470799.

Immunogen: Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2; NP_006522.2. Epitope: C Terminus

Note: This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2)

Jiang ST, Chiou YY, Wang E, Chien YL, Ho HH, Tsai FJ, Lin CY, Tsai SP, Li H.; Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.; Hum Mol Genet.2009 May 1;18(9): 1566-77.; Pubmed 19208653

28558993

NP_783195.2

NM_175605.4

92,381 Da

Assembly Of The Primary Cilium Pathway (1268846); Endochondral Ossification Pathway (198812); Hedgehog 'off' State Pathway (1269636); Hedgehog Signaling Events Mediated By Gli Proteins Pathway (137912); Intraflagellar Transport Pathway (1268853); Organelle Biogenesis And Maintenance Pathway (1268838); Signal Transduction Pathway (1269379); Signaling By Hedgehog Pathway (1269633)

This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

TTC10: Involved in primary cilium biogenesis. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Unknown function. Chromosomal Location of Human Ortholog: 13q12.1. Cellular Component: centriole; cilium. Molecular Function: protein binding

0.1毫克

300美元