抗体

Goat anti-14-3-3 sigma/Stratifin Antibody

MBS421444

0.1毫克

300美元

多克隆

山羊

未共轭

人类, 小鼠, 大鼠, 狗

免疫印迹, 免疫组化, 酶免疫法

抗体

Goat anti-14-3-3 sigma/Stratifin Antibody

14-3-3 sigma/Stratifin

SFN; 14-3-3sigma; stratifin; HGNC: 10773; YWHAS; 14-3-3sigma antibody; stratifin antibody; HGNC: 10773 antibody; SFN antibody; YWHAS antibody; 14-3-3 sigma / Stratifin

14-3-3 protein sigma; 14-3-3 protein sigma; 14-3-3 protein sigma; stratifin; Epithelial cell marker protein 1; Stratifin

SFN

14-3-3 sigma; Stratifin

SFN;SFN;YWHAS;HME1

1433S_HUMAN

多克隆

山羊

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

248

DLHTLSEDSYKDST

通过硫酸铵沉淀和用免疫多肽抗原亲和层析从山羊血清中纯化得到的

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

肽酶联免疫吸附测定(EIA), 免疫印迹(免疫印迹), 免疫组化(免疫组化)

Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4ug/ml. Western Blot: Approx.28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0. 03-0.1ug/ml.

Immunogen: Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1. Epitope: Internal region

5454052

NP_006133.1

NM_006142.3

24,336 Da

Activation Of BAD And Translocation To Mitochondria Pathway (1270271); Activation Of BH3-only Proteins Pathway (1270270); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (1270262); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (1269741); Cell Cycle Checkpoints Pathway (1269742); Cell Cycle Pathway (83054)

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 1p36.11. Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; extracellular space; mitochondrion; nucleus. Molecular Function: phosphoprotein binding; protein binding; protein domain specific binding; protein kinase binding; protein kinase C inhibitor activity. Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction resulting in induction of apoptosis; keratinization; negative regulation of caspase activity; negative regulation of protein kinase activity; positive regulation of cell growth; positive regulation of epidermal cell differentiation; positive regulation of protein export from nucleus; regulation of cyclin-dependent protein kinase activity; regulation of epidermal cell division; release of cytochrome c from mitochondria; signal transduction

0.1毫克

300美元