TJP1; tight junction protein 1 (zona occludens 1); DKFZp686M05161; MGC133289; ZO-1; OTTHUMP00000174520; tight junction protein 1; tight junction protein ZO-1; zona occludens 1; zonula occludens 1 protein; TJP1 antibody; tight junction protein 1 (zona occludens 1) antibody; DKFZp686M05161 antibody; MGC133289 antibody; ZO-1 antibody; OTTHUMP00000174520 antibody; tight junction protein 1 antibody; tight junction protein ZO-1 antibody; zona occludens 1 antibody; zonula occludens 1 protein antibody
tight junction protein ZO-1 isoform a; Tight junction protein ZO-1; tight junction protein ZO-1; tight junction protein 1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1
Tested: Human; Expected from sequence similarity: Human
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
100ug specific antibody in 200ul
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 170kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kDa according to NP_783297.2). Recommended concentration: 0.3-1ug/ml.
Immunogen: Peptide with sequence C-PKPTSQNQFSEHDKT, from the internal region of the protein sequence according to NP_003248.3; NP_783297.2. Epitope: Internal region
Note: This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2)
NCBI GI登录号 :
Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Apoptosis Pathway (1270262); Apoptotic Cleavage Of Cell Adhesion Proteins Pathway (1270290); Apoptotic Cleavage Of Cellular Proteins Pathway (1270288); Apoptotic Execution Phase Pathway (1270287); E-cadherin Signaling In The Nascent Adherens Junction Pathway (138005); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (83102); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (515); Gap Junction Pathway (83072)
This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014]
ZO1: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with tight junctions (TJ). May mediate dynamic changes in the composition of TJs. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting the protein kinase MRCKb to the leading edge of migrating cells. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Forms homodimers and heterodimers with ZO-2 or ZO-3. Interacts with occludin, CALM, claudins, CGN, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with MRCKb and MYZAP. Interacts (via PDZ domain) with Cx43. ZO-2 and ZO-3 shuttle between the TJ and the nucleus, where they may regulate gene expression. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins can be up- or downregulated in cancer and are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Alternative splicing produces two isoforms. The long isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 15q13. Cellular Component: apical junction complex; apical part of cell; apical plasma membrane; apicolateral plasma membrane; basolateral plasma membrane; cell junction; cell-cell adherens junction; cytoplasm; cytosol; gap junction; intercellular canaliculus; nucleus; plasma membrane; tight junction. Molecular Function: calmodulin binding; protein binding; protein domain specific binding. Biological Process: blastocyst formation; intercellular junction assembly; sensory perception of sound
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308