抗体

EGFRvIII (Epidermal Growth Factor Receptor, Variant III)

MBS4380546

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

单克隆

兔

未共轭

[GFR/2600R]

抗体

EGFRvIII (Epidermal Growth Factor Receptor, Variant III)

[EGFRvIII (Epidermal Growth Factor Receptor, Variant III)]

[Erbb1 variant III; ERBB1 variant III; HER1 variant III; Proto-oncogene c-ErbB-1 variant III; Receptor Tyrosine Protein Kinase variant III; ErbB1 variant III]

[epidermal growth factor receptor isoform a; Epidermal growth factor receptor; epidermal growth factor receptor; epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1]

[表皮生长因子受体]

[表皮生长因子受体;表皮生长因子受体;ERBB;HER1;mENA;ERBB1;PIG61;NISBD2;ERBB;ERBB1;HER1]

单克隆

IgG

[GFR/2600R]

兔

Human. Others not known.

405

EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistology (Formalin-Fixed)

Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

免疫组化(免疫组化)

免疫组化(免疫组化)

SDS-Page

Immunogen: KLH conjugate of EGFRvIII-specific, 14-amino acid peptide from the predicted amino acid sequence at the fusion junction (LEEKKGNYVVTDHC). Positive Control: A431 Cells. Placenta, Breast, Colon or Bladder Carcinoma

Cellular Localization: Cell Surface. Chromosome Location: 7p11.2

重组兔单克隆抗体

Wikstrand CJ, et al. Cancer Res. 1995 Jul 15; 55(14):3140-8.

29725609

NP_005219.2

NM_005228.4

P00533

~145kDa (vIII variant)

AGE/RAGE Pathway (698754); ARMS-mediated Activation Pathway (1269471); Adaptive Immune System Pathway (1269171); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Androgen Receptor Signaling Pathway (198806); Arf6 Signaling Events Pathway (138034); Aryl Hydrocarbon Receptor Pathway (1458217); Aryl Hydrocarbon Receptor Pathway (1458288); Association Between Physico-Chemical Features And Toxicity Associated Pathways (1458235)

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Plays a role in enhancing learning and memory performance ().

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340