抗体

CD3e(T细胞标记)

MBS438059

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠, 狗, 鸡, 牛, 黑猩猩, 猕猴

免疫印迹, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪

抗体

CD3e(T细胞标记)

[CD3e]

[CD 3E; CD3 epsilon; CD3 TCR complex; CD3E; CD3e antigen epsilon polypeptide (TiT3 complex); T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex]

[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; CD3e molecule, epsilon (CD3-TCR complex); T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e]

[CD3E]

[CD3E;CD3E;T3E;TCRE;IMD18;T3E]

CD3E_HUMAN

多克隆

免疫球蛋白

兔

Human. Predict reacted with: Elephant, gibbon, Guinea Pig, Hamster, Horse, Macaque, Mole Rat, Monkey, Mouse, New World monkey, Orangutan, Pig, Rabbit, Rat, Rhesus Monkey, Sheep, Ape, Cat, Chicken, Chimpanzee, Cow, Dog, Frog, Opossum.

207

Recognizes the chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Supplied as 200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available Without BSA at 1.0mg/ml.

Antibody with azide-Store at 2 to 8 degree C. Antibody without azide-store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

流式细胞仪(FC/流式细胞仪), 免疫荧光(IF), 免疫印迹(免疫印迹), 免疫沉淀(免疫沉淀)

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (1-2ug/ml). Western Blotting (1-2ug/ml for 60 minutes at RT). Immunoprecipitation (1-2ug/500ug protein lysate). Optimal dilution for a specific application should be determined.

试验数据

免疫印迹

Localization: Cell surface and cytoplasmic. Positive Control: Jurkat cells. Tonsil and lymph node. Human Chromosomal Location: 11q23.3

Immunogen: A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3e. Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide

1. Cibull ML et. al. Histopathology, 1989, 15(6):599-605. 2. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.

4502671

NP_000724.1

NM_000733.3

P07766

20kDa

Adaptive Immune System Pathway (366160); CXCR4-mediated Signaling Events Pathway (137910); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (119552); Downstream TCR Signaling Pathway (106418); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Generation Of Second Messenger Molecules Pathway (106417); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane. Molecular Function: protein binding; receptor signaling complex scaffold activity; transmembrane receptor activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; protein kinase binding; SH3 domain binding. Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; regulation of apoptosis; positive regulation of interleukin-4 production; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of T cell proliferation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient. Disease: Immunodeficiency 18

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA at 1mg/ml)

340