产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
CD86 (Dendritic Cells Maturation Marker) Mouse Monoclonal Antibody
目录 :
MBS438121
规格 :
0.02 mg (With BSA & Azide at 0.2 mg/ml)
价格 :
190美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
[BU63]
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS438121 图像 1
Formalin-fixed, paraffin-embedded human Esophageal Tumor stained with CD86 Monoclonal Antibody (BU63).
图像 2 :
MyBioSource MBS438121 图像 2
Flow Cytometric Analysis of human PBMC's Cells. usingCD86 Mouse Monoclonal Antibody (BU63); Goat anti-mouse IgG-CF488 (red); Isotype Control (green).
图像 3 :
MyBioSource MBS438121 图像 3
Flow Cytometric Analysis of PFA fixed Ramos Cells. CD86 Mouse Monoclonal Antibody (BU63). followed byGoat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
产品信息
目录号 :
MBS438121
产品类型 :
抗体
产品全称 :
CD86 (Dendritic Cells Maturation Marker) Mouse Monoclonal Antibody
产品简称 :
[CD86]
产品名称同义词 :
[Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2]
其他名称 :
[T-lymphocyte activation antigen CD86 isoform 4; T-lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; CD86 molecule; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD_antigen: CD86]
产品基因名称 :
[CD86]
其他基因名称 :
[CD86;CD86;B70;B7-2;B7.2;LAB72;CD28LG2;CD28LG2]
UniProt数据库进入名 :
CD86_HUMAN
克隆性 :
单克隆
抗体亚型 :
IgG1, kappa
克隆 :
[BU63]
宿主 :
小鼠
反应物种 :
人类, 小鼠, 大鼠
序列长度 :
217
特异性 :
Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
形式 :
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
储存稳定性 :
Antibody with azide - store at 2-8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
检测过的应用 :
Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin
应用笔记 :
Functional Studies (Order Ab without Azide). Flow Cytometry (1-2ug/million cells in 0.1ml). Immunofluorescence (0.5-1ug/ml). Western Blot (0.5-1ug/ml). Immunoprecipitation (0.5-1 -g/500ug protein Lysate). Immunohistology (Formalin-fixed) (2-4ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.
image1头 :
免疫组化(免疫组化)
image2头 :
流式细胞仪(FC/流式细胞仪
image3头 :
流式细胞仪(FC/流式细胞仪)
image4头 :
免疫荧光(IF)
image4描述 :
mmunofluorescencestaining of PFA fixed Ramos cells using CD86 Mouse Monoclonal Antibody (BU63) followed by goat anti-mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
image5头 :
十二烷基磺酸钠-PAGE
image5描述 :
SDS-PAGE Analysis Purified CD86 Mouse Monoclonal Antibody (BU63) Confirmation of Integrity and Purity of Antibody.
其它信息1 :
Cellular Localization: Cell Surface. Immunogen: ARH-77 (B-lymphoblastoid cell line)
其他信息2 :
Hu-Chromosome Location: 3q13.33. Positive Control: 293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.
产品引用 :
Engel P et. al. Blood. 1994;84(5):1402-7.Caux C et. al. J Exp Med. 1994;180(5):1841-7. Mauri D et. al. J Immunol. 1995;155(1):118-27.Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)
NCBI GI登录号 :
332634950
NCBI登录号 :
NP_001193853.1
NCBI基因登录号 :
NM_001206924.1
UniProt数据库登录号 :
P42081
NCBI分子量 :
70kDa
NCBI信号通路 :
Adaptive Immune System Pathway (1269171); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (1269178); CD28 Dependent PI3K/Akt Signaling Pathway (1269179); CD28 Dependent Vav1 Pathway (1269180); CTLA4 Inhibitory Signaling Pathway (1269181)
NCBI总结 :
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
UniProt数据库总结 :
CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Immunoglobulin superfamily; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q21. Cellular Component: cell surface; integral to membrane; plasma membrane. Molecular Function: phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; receptor activity. Biological Process: cell-cell signaling; immune response; phosphoinositide-mediated signaling; positive regulation of cell proliferation; regulation of phosphoinositide 3-kinase cascade; T cell activation; T cell costimulation
尺寸1 :
0.02 mg (With BSA & Azide at 0.2 mg/ml)
价格1 :
190美元
尺寸2 :
0.1 mg (With BSA & Azide at 0.2mg/ml)
价格2 :
340
size3 :
0.1 mg (Without BSA & Azide at 1mg/ml)
价格3 :
340
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。