抗体

CD68(巨噬细胞标记)小鼠单克隆抗体

MBS438131

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

单克隆

小鼠

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

抗体

CD68(巨噬细胞标记)小鼠单克隆抗体

[CD68]

[GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1]

[macrosialin isoform B; Macrosialin; macrosialin; CD68 molecule; Gp110; CD_antigen: CD68]

[CD68]

[CD68;CD68;GP110;LAMP4;SCARD1]

CD68_HUMAN

m

IgG1, kappa

小鼠

Human, Monkey, Mouse, and Rat. Others not known.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

存放在2-8°C

Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC), Immunohistology (Formalin-fixed)

Western blotting (1-2ug/ml). Immunofluorescence (1-2ug/ml). Flow Cytometry (1-2ug/million cells). Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) . (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

免疫组化(免疫组化)

免疫组化(免疫组化)

十二烷基磺酸钠-PAGE

免疫印迹(免疫印迹)

Western Blot Analysis of human Spleen tissue lysate using CD68 Monoclonal Antibody (C68/684).

流式细胞仪(FC)

Flow Cytometric Analysis of U87MG cells using CD68 Monoclonal Antibody (C68/684); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Human Unigene: 647419. Hu-Chromosome Location: 17p13.1. Immunogen: Recombinant human CD68 protein. Cellular Localization: Cell Surface and Cytoplasmic. Positive Control: U87MG cells,Tonsil, Lymph Node or Spleen

This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Pulford KA et. al. Journal of Clinical Pathology, 1989, 42(4):414-21. Warnke RA et. al. Am J of Pathol, 1989, 135:1089-95.

91199550

NP_001035148.1

NM_001040059.1

P34810

110kDa

溶体通路(99052);溶体通路(96865)

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: membrane

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340