抗体

GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody

MBS438192

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

单克隆

小鼠

未共轭

[SPM507]

人类, 小鼠, 大鼠, 鸡, 牛

免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 流式细胞仪

抗体

GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody

[GFAP]

[星形胶质细胞或中间丝蛋白类, 胶质纤维酸性蛋白]

[glial fibrillary acidic protein isoform 2; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein]

[GFAP]

[GFAP;GFAP;ALXDRD;GFAP]

GFAP_HUMAN

单克隆

IgG1

[SPM507]

小鼠

Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken. Others not known.

431

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

流式细胞仪(FC/流式细胞仪), 免疫荧光(IF), 免疫印迹(免疫印迹), 免疫组化(免疫组化)福尔马林

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (1-2ug/ml). Western Blotting (0.5-1ug/ml). Immunohistology (Formalin-fixed) (0.5-1 ug/ml for 30 minutes at RT) . (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

免疫组化(免疫组化)

免疫印迹(免疫印迹)

十二烷基磺酸钠-PAGE

Cellular Localization: Cytoplasmic. Immunogen: GFAP isolated from pig spinal cord

Hu-Chromosome Location: 17q21.31. Positive Control: Brain or Astrocytoma

Herpers MJ et. Al. 1986, Acta Neuropathol, 70:333-339. Van Muijen GN et. al. 1987, Lab Invest, 57:359-369. Debus E, et. al. 1983, Differentiation, 25(2):193-203

196115290

NP_001124491.1

NM_001131019.2

P14136

~50kDa

Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neural Crest Differentiation Pathway (672460); Nuclear Signaling By ERBB4 Pathway (1269499); Signal Transduction Pathway (1269379); Signaling By ERBB4 Pathway (1269496); Spinal Cord Injury Pathway (739007)

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: cytoplasm; cytosol; intermediate filament cytoskeleton; lysosome. Molecular Function: protein binding. Biological Process: regulation of protein complex assembly. Disease: Alexander Disease

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340