产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
纤维连接蛋白
目录 :
MBS438210
规格 :
0.02 mg (With BSA & Azide at 0.2 mg/ml)
价格 :
190美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
[TV-1]
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 流式细胞仪, 酶免疫法
更多信息或购买 :
图像
图像 1 :
MyBioSource MBS438210 图像 1
Formalin-fixed, paraffin-embedded human Pancreatic Adenocarcinoma stained with Fibronectin Monoclonal Antibody (TV-1)
产品信息
目录号 :
MBS438210
产品类型 :
抗体
产品全称 :
纤维连接蛋白
产品简称 :
[纤维连接蛋白]
产品名称同义词 :
[Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3]
其他名称 :
[fibronectin isoform 3 preproprotein; Fibronectin; fibronectin; cold-insoluble globulin; migration-stimulating factor; fibronectin 1; Cold-insoluble globulin; CIG]
产品基因名称 :
[FN1]
其他基因名称 :
[FN1;FN1;FN;CIG;FNZ;MSF;ED-B;FINC;GFND;LETS;GFND2;FN;FN;CIG]
UniProt数据库进入名 :
FINC_HUMAN
克隆性 :
单克隆
抗体亚型 :
IgG1, kappa
克隆 :
[TV-1]
宿主 :
小鼠
反应物种 :
Human, Pig, Mouse and Rat. Others-not known.
序列长度 :
2355
特异性 :
Fibronectin is a soluble dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. This MAb reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this MAb localizes to tumor vessels where it binds to the underlying basement. Epitope recognized by this antibody is not accessible in normal tissues to the circulating MAb indicating that it can be used to specifically target tumor vessels in vivo. TV-1 is reportedly useful for delivering vasoactive agents to tumors to induce increased vascular permeability or blood flow prior to treatment with chemotherapeutic drugs or MAbs.
形式 :
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
储存稳定性 :
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
检测过的应用 :
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)
应用笔记 :
Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (0.5-1ug/ml) Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.
image1头 :
免疫组化
其它信息1 :
Cellular Localization: Connective tissue matrix. Positive Control: SW156 cells or Kidney. Human Chromosomal Location: 2q35
其他信息2 :
Immunogen: T-cell lymphoma biopsy. Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide. Supplied As: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
产品引用 :
1. Epstein AL, et. al. Cancer Research, 1995, 55(12):2673-80.
NCBI GI登录号 :
16933542
NCBI登录号 :
NP_002017.1
NCBI基因登录号 :
NM_002026.2
UniProt数据库登录号 :
P02751
NCBI分子量 :
220kDa (monomer); 440kDa (dimer)
NCBI信号通路 :
Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cell Surface Interactions At The Vascular Wall Pathway (106062); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Fibronectin Matrix Formation Pathway (771601)
NCBI总结 :
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q34. Cellular Component: extracellular matrix; extracellular space; apical plasma membrane; fibrinogen complex; ER-Golgi intermediate compartment; extracellular region; basal lamina. Molecular Function: collagen binding; heparin binding; integrin binding; protein binding; protease activator activity; protease binding. Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; extracellular matrix disassembly; regulation of cell shape; platelet degranulation; calcium-independent cell-matrix adhesion; cell-substrate junction assembly; acute-phase response; response to wounding; peptide cross-linking; angiogenesis; cell adhesion; blood coagulation; leukocyte migration. Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency
尺寸1 :
0.02 mg (With BSA & Azide at 0.2 mg/ml)
价格1 :
190美元
尺寸2 :
0.1 mg (With BSA & Azide at 0.2mg/ml)
价格2 :
340
size3 :
0.1 mg (Without BSA & Azide at 1mg/ml)
价格3 :
340
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。