抗体

Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody

MBS438228

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

单克隆

小鼠

未共轭

[VM452]

人类, 小鼠, 大鼠, 狗, 鸡, 牛

免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 流式细胞仪

抗体

Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody

[弹性蛋白]

[弹性蛋白]

[弹性蛋白;弹性蛋白;弹性蛋白;弹性蛋白]

[弹性蛋白]

[弹性蛋白;弹性蛋白;HEL113;CTRCT30]

VIME_HUMAN

单克隆

IgG1

[VM452]

小鼠

Human, Cow, Dog, Cat, Pig, Goat, Chicken. Does not react with Mouse and Rat

466

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

流式细胞仪(FC/流式细胞仪), 免疫荧光(IF), 免疫印迹(免疫印迹), 免疫组化(免疫组化)福尔马林

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). Western Blot (0.25-0.5ug/ml). Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

免疫印迹(免疫印迹)

免疫组化(免疫组化)

免疫印迹(免疫印迹)

十二烷基磺酸钠-PAGE

SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VM452). Confirmation of Integrity and Purity of Antibody.

流式细胞仪(FC)

Flow Cytometry of human Vimentin on Jurkat Cells, Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Mouse Monoclonal Antibody (VM452).

Cellular Localization: Cytoplasmic. Immunogen: Recombinant full-length human vimentin protein

Hu-Chromosome Location: 10p12.33. Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143

62414289

NP_003371.2

NM_003380.3

P08670

57-60kDa

Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (1270262); Apoptotic Cleavage Of Cellular Proteins Pathway (1270288); Apoptotic Execution Phase Pathway (1270287); Aurora B Signaling Pathway (138080); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1270289); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852705)

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190美元

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340