抗体

AKT(C20)抗体

MBS440053

0.2毫克

240美元

多克隆

兔

未共轭

人类, 小鼠

免疫印迹, 免疫组化

抗体

AKT(C20)抗体

AKT

Akt, partial; Akt; Akt

AKT

Akt

Q66XT6_ANOST

多克隆

兔

小鼠, 大鼠, 人类

286

小鼠, 大鼠, 和人类AKT

200微克/毫升兔多克隆IgG在1毫升PBS含0.1% 叠氮化钠和0.2% 明胶

0.200毫克/毫升

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

免疫印迹, 免疫组化(免疫组化)

Origin: Akt1 (C20) is provided as an affinity purified rabbit polyclonal antibody, raised against a synthetic peptide mapping to the carboxy terminal domain of human Akt1.

Immunogen: Synthetic peptide mapping to the carboxy terminal domain of human AKT1. Differs from corresponding mouse sequence by one residue.

Akt1, also known as Protein kinase B, is a serine/threonine kinase that is at the center of many signal transduction pathways. Akt1 regulates glucose metabolism, transcription, apoptosis, cell proliferation, angiogenesis, and cell motility (1). The activation of Akt1 occurs downstream of PI-3K, which upon activation by an extracellular molecule, generates PIP (3) (2). PIP (3) is a lipid second messenger that promotes the translocation of Akt1 to the plasma membrane via PH (pleckstrin-homology) domain binding (2-4). At the plasma membrane Akt1 is phosphorylated and activated by PDK-1 at its catalytic site on residue Thr308 (1). Residue Ser473 is also phosphorylated at this time but the kinase has not yet been identified (1). In this activated form Akt1 phosphorylates proteins that contain the motif, R-x-R-x-x-S/T-F/L, at the plasma membrane, in the cytosol, and in the nucleus (1). In addition to its kinase activity, Akt1 forms complexes with other proteins that are not substrates. These other proteins act as modulators of Akt1 activity (1).

1. Brazil DP, Park J, Hemmings BA. 2002. PKB binding proteins. Getting in on the Akt. Cell 111 (3):293-303.2. Nicholson KM, Anderson NG. 2002. The protein kinase B/Akt signalling pathway in human malignancy. Cell Signal 14(5): 381-395.3. Marte BM, Downward J. 1997. PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond. Trends Biochem Sci 22(9): 355-358.4. Coffer PJ, Jin J, Woodgett JR. 1998. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 335(Pt1): 1-13.

51539256

AAU06122.1

32,995 Da

0.2毫克

240美元