抗体

Rabbit anti TGFBR1 (Non-Phosphospecific) Polyclonal Antibody

MBS460587

0.1毫克

285美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 流式细胞仪, 流式细胞仪, 酶免疫法

抗体

Rabbit anti TGFBR1 (Non-Phosphospecific) Polyclonal Antibody

[TGFBR1]

[转化生长因子β受体1(TGFbR1)]

[TGF-beta receptor type-1 isoform 3; TGF-beta receptor type-1; TGF-beta receptor type-1; transforming growth factor beta receptor 1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5; ALK-5; ALK5; Serine/threonine-protein kinase receptor R4; SKR4; TGF-beta type I receptor; Transforming growth factor-beta receptor type I; TGF-beta receptor type I; TbetaR-I]

[TGFBR1]

[TGFBR1; TGFBR1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4; tbetaR-I; ALK5; SKR4; TGFR-1; ALK-5; ALK5; SKR4; TGF-beta receptor type I; TbetaR-I]

多克隆

IgG

兔

人类, 小鼠, 大鼠

507

This antibody recognizes TGFbetaR1protein at phosphorylation sites of Serine 187, 189, and/or 191 This antibody reacts with human, rat and mouse. The other species are not tested.

This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer

The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

酶联免疫吸附测定(EIA), 流式细胞仪(FC/流式细胞仪), 免疫组化(免疫组化), 免疫沉淀(免疫沉淀), 免疫印迹(免疫印迹)

ELISA: 0.01-0.1 ug/ml. Flow Cytometry: Not tested. Immunohistochemistry: 2-10 ug/ml. Immunoprecipitation: 2-5 ug/ml. Western Blot: 0.1-1 ug/ml. Optimal dilutions should be determined by researchers for the specific applications

免疫印迹

Immunohistrochemistry

Antigen Preparation: A synthetic peptide derived from internal sequence (-TSGSFSFLP-) without any phosphorylation among these Serines of TGFbeta receptor type-1. This sequence is also identical within human, rat, mouse, chicken and other species.

Positive Control: MCF7. Cellular Location: Membrane/Qytoplasm

Transforming growth factor beta isoforms (TGF-beta1, 2, 3) play vital roles in regulating cellular growth and differentiation, and they signal through a highly restricted subset of receptors known as TGF-betatype I receptor (TbetaR-I) and TGF-beta type II receptor (TbetaR-II). Expression of T beta R-I is more ubiquitous than that of T beta R-IL There was no staining of T beta R-II in the central nervous system, while intense T beta R-I staining was found specifically in nervous tissues. Expression of T beta R-I and T beta R-II was mostly coine dent with that of their ligands, suggesting that TGF-beta s act as multiple mediators during organogenesis. TGF-beta binds with high affinity to its receptor, a transmembrane protein with a cytoplasmic serine/threonine kinase domain, to form a dynamic dimer with phosphorylation in the cytoplasmic SerlThr residuals which cause its kinase activity and association with another TGF beta-binding protein, the type I receptor, to signal growth inhibition and early gene responses.

817050443

NP_001293139.1

NM_001306210.1

P36897

~55kDa

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); ALK1 Signaling Events Pathway (137968); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (83106)

The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.

0.1毫克

285美元