抗体

小鼠抗磷酸甘油醛脱氢酶抗体

MBS462002

0.1毫克

350美元

单克隆

小鼠

未共轭

6C5

人类, 小鼠, 大鼠, 狗

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀

抗体

小鼠抗磷酸甘油醛脱氢酶抗体

磷酸甘油醛脱氢酶

小鼠抗磷酸甘油醛脱氢酶

Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; BARS-38; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH

磷酸甘油醛脱氢酶

Gapdh; Gapdh; Gapd; Gapd

G3P_RAT

单克隆

6C5

小鼠

人类, 大鼠, 小鼠, 狗, 鱼类

This antibody recognizes Glyceraldehyde-3-PDH monomer (36 kDa) and the dimer form. It reacts with human, rabbit, rat, mouse, dog, cat, fish and pig. It does not react with GAPDH from bovine, goat and yeast.

The Mouse IgG1 is purified by Epitope Affinity Purification

This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.

Size: 100 ug/200 ul

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

酶联免疫吸附测定, 免疫印迹, 免疫组化, 免疫沉淀, 免疫荧光

Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml

Antigen Preparation: A purified rabbit muscle GAPDH (whole molecule).

Positive Control: Rabbit Muscle. Cellular Location: Cytoplasmic

122065190

P04797

36 kDa (monomer)

Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Androgen Receptor Signaling Pathway (198529); Glycolysis / Gluconeogenesis Pathway (83319); Glycolysis / Gluconeogenesis Pathway (287); Glycolysis And Gluconeogenesis Pathway (198445); Metabolic Pathways (132933)

catalyzes the interconversion of D-glyceraldehyde 3-phosphate, phosphate, and NAD(+) to 3-phospho-D-glyceroyl phosphate and NADH; may contribute to apoptosis [RGD]

Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Ref.3 Ref.13 Ref.15. Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Subunit structure: Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct. By similarity. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Ref.13 Ref.14 Ref.15. Subcellular location: Cytoplasm cytosol. Nucleus. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Ref.13 Ref.14 Ref.15. Tissue specificity: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Ref.9. Post-translational modification: S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus. The effect of S-nitrosylation on enzymatic activity is unclear: according to some authors, it inhibits enzymatic activity and increases endogenous ADP-ribosylation, inhibiting the enzyme in a non-reversible manner (Ref.12). According to others, it does not affect glycolysis (Ref.13). ADP-ribosylation is likely to be a pathophysiological event associated with inhibition of gluconeogenesis.ISGylated. Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

0.1毫克

350美元