产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
兔抗STAT3
目录 :
MBS462112
规格 :
0.1毫克
价格 :
270美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类, 小鼠, 大鼠, , ,
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化
更多信息或购买 :
产品信息
目录号 :
MBS462112
产品类型 :
抗体
产品全称 :
兔抗STAT3
产品简称 :
STAT3
产品名称同义词 :
Rabbit anti-Phospho-STAT3; Signal transducer and activator of transcription 3 (STAT3)
其他名称 :
signal transducer and activator of transcription 3 isoform 1; Signal transducer and activator of transcription 3; signal transducer and activator of transcription 3; OTTHUMP00000198123; OTTHUMP00000198128; DNA-binding protein APRF; acute-phase response factor; signal transducer and activator of transcription 3 (acute-phase response factor); Acute-phase response factor
产品基因名称 :
STAT3
其他基因名称 :
STAT3;STAT3;APRF;HIES;FLJ20882;MGC16063;APRF
UniProt数据库进入名 :
STAT3_HUMAN
宿主 :
反应物种 :
人类, 大鼠, 小鼠, 狗, 牛, 鸡
特异性 :
This antibody recognizes STAT3 without a phosphorylated sites of Ser 727.
纯度 :
The Rabbit IgG is purified by Epitope -Affinity Purification.
形式 :
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
浓度 :
Size: 100 ug/200 ul
储存稳定性 :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
检测过的应用 :
酶联免疫吸附测定, 免疫印迹, 免疫组化
应用笔记 :
Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 1:50 ug/ml
其它信息1 :
Antigen Preparation: A synthetic peptide surrounding to the epitope -PMSPR-- without phosphorylation sites at Ser727 of STAT3 protein from human, mouse, rat, chicken dog and bovine origins.
其他信息2 :
Positive Control: A431 whole cell lysate. Cellular Location: Nuclear
产品描述 :
STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. The phosphorylation of STAT3 induces STAT dimerization, nuclear translocation and DNA binding. Stat3 is constitutively activated in a number of human tumors. Stat3- activated transcription seems to be regulated by serine phosphorylation at Ser727.
产品引用 :
Ritsuko Matsuo et al. A new expression cloning strategy for isolation of substrate-specific kinases by using phosphorylation site-specific antibody. Journal of Immunological Methods. Volume 247, Issues 1-2, 1 January 2001, Pages 141-151
NCBI GI登录号 :
21618340
NCBI登录号 :
NP_644805.1
NCBI基因登录号 :
NM_139276.2
UniProt数据库登录号 :
P40763
NCBI分子量 :
94 kDa
NCBI信号通路 :
Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Androgen Receptor Signaling Pathway (198806); B Cell Receptor Signaling Pathway (198909); CXCR4-mediated Signaling Events Pathway (137910); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864)
NCBI总结 :
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]
UniProt数据库总结 :
Function: Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Ref.10. Subunit structure: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1 and TMF1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts (via SH2 domain) with NLK. Interacts with ARL2BP; the interaction is enhanced by LIF and JAK1 expression. By similarity. Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import. By similarity. Interacts with CAV2; the interaction is increased on insulin-induced tyrosine phosphorylation of CAV2 and leads to STAT3 activation. By similarity. Interacts with ARL2BP; interaction is enhanced with ARL2. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.20. Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Ref.16 Ref.20. Tissue specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Post-translational modification: Tyrosine phosphorylated upon stimulation with EGF. By similarity. Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Ref.6 Ref.14 Ref.15 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24. Involvement in disease: Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [. MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures. Ref.26 Ref.27. Miscellaneous: Involved in the gp130-mediated signaling pathway. Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.
尺寸1 :
0.1毫克
价格1 :
270美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。