产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
兔抗孕酮受体抗体
目录 :
MBS462117
规格 :
0.1毫克
价格 :
350美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠,
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化
更多信息或购买 :
产品信息
目录号 :
MBS462117
产品类型 :
抗体
产品全称 :
兔抗孕酮受体抗体
产品简称 :
孕激素受体
产品名称同义词 :
兔抗PR
其他名称 :
progesterone receptor; Progesterone receptor; progesterone receptor; OTTHUMP00000236114; OTTHUMP00000236115; OTTHUMP00000236117; OTTHUMP00000236119; nuclear receptor subfamily 3 group C member 3; progesterone receptor; Nuclear receptor subfamily 3 group C member 3
其他基因名称 :
PGR;PGR;PR;NR3C3;NR3C3
UniProt数据库进入名 :
PRGR_HUMAN
宿主 :
反应物种 :
人类, 小鼠, 大鼠, 牛, 仓鼠
特异性 :
This antibody recognizes ~116 kDa of human PR protein. The other species are not tested
纯度 :
The Rabbit IgG is purified by Epitope Affinity Purification.
形式 :
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
浓度 :
Size: 100 ug/200 ul
储存稳定性 :
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. Avoid repeated freezing-thawing cycles.
检测过的应用 :
酶联免疫吸附测定, 免疫印迹, 免疫组化
应用笔记 :
Western Blot: 0.1-1 ug/ml. ELISA: 0.01-0.1 ug/ml. Immunoprecipitation: 2-5 ug/ml. Immunohistochemistry: 1-2 ug/ml
其它信息1 :
Antigen Preparation: A synthetic peptide corresponding to C-terminus of human Progesterone receptor.
其他信息2 :
Positive Control: Breast Cancer Tissue or MCF-7 cell. Cellular Location: Nuclear
产品描述 :
The progesterone receptor (PR) is a member of the steroid receptor superfamily. PR expression indicates a responsive estrogen receptor (ER) pathway, and therefore, may predict likely response to endocrine therapy in human breast cancer. In humans, the progesterone receptor (PR) gene gives rise to multiple isoforms. The "B" (PR-B, 116kDa, 933aa) contains a proline-rich N-term (aa 1 - 566), a central DNA-binding domain (DBD) (aa 567 - 636), a nuclear localization motif (aa 637 - 644), and a hormone binding/dimerization domain (HBD) (aa 645 - 933). PR-A (94 kDa, 769aa) utilizes a different start site that shortens the N-terminus by 164 amino acids. The N-terminus in both is rich in serine that is phosphorylated in response to hormone binding. In the absence of hormone, a few PR-A and -B molecules are phosphorylated at Serine 190 (S190). Hormone increases this number two-fold, providing evidence for hormone stimulation. The common Serine at 294 can only be phosphorylated on PR-B, due to a difference in N-terminal conformation. This may account for functional differences between the molecules. Alternate start sites also generate two shorter forms that lack the N-terminus: PR-C (60 kDa, 339 aa), PR-M (38 kDa, 314aa). PR-A, -B and -C are known to heterodimerize. Alternate splicing of PR-A generates at least four other isoforms. All contain aa 1 - 516 (with the N-terminus), and are either truncated or show a partial deletion of the HBD.
产品引用 :
Zheng ZY, Lin VC. Anti-estrogenic effect of unliganded progesterone receptor is estrogen-selective in breast cancer cells MCF-7. Cancer Lett. 2008 May 12 (in press)
NCBI GI登录号 :
189935
UniProt数据库登录号 :
P06401
NCBI分子量 :
116 kDa
NCBI信号通路 :
Cellular Roles Of Anthrax Toxin Pathway (138076); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); Oocyte Meiosis Pathway (126909); Oocyte Meiosis Pathway (126872); Ovarian Infertility Genes Pathway (198801); Progesterone-mediated Oocyte Maturation Pathway (119304); Progesterone-mediated Oocyte Maturation Pathway (503)
NCBI总结 :
This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq]
UniProt数据库总结 :
Function: The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. Ref.16 Ref.17 Ref.19 Ref.20 Ref.21 Ref.22 Ref.24Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation. Ref.16 Ref.17 Ref.19 Ref.20 Ref.21 Ref.22 Ref.24. Subunit structure: Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. Interacts with PRMT2. Ref.14 Ref.15 Ref.18 Ref.19 Ref.20 Ref.24. Subcellular location: Nucleus. Cytoplasm. Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases. Ref.16 Ref.17 Ref.22Isoform A: Nucleus. Cytoplasm. Note: Mainly nuclear. Ref.16 Ref.17 Ref.22. Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Post-translational modification: Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G2/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.16 Ref.17 Ref.21 Ref.22 Ref.23 Ref.24Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294. Ref.19 Ref.20 Ref.21Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294. Sequence similarities: Belongs to the nuclear hormone receptor family. NR3 subfamily.Contains 1 nuclear receptor DNA-binding domain.
尺寸1 :
0.1毫克
价格1 :
350美元
更多信息或购买 :
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。