C-Raf (N-terminal Region) | MyBioSource MBS470008 产品信息

产品简要

公司名称 :

MyBioSource

产品类型 :

抗体

产品名称 :

C-Raf (N-terminal Region)

MBS470008

规格 :

0.1毫升

价格 :

280美元

克隆性 :

单克隆

宿主 :

小鼠

共轭标签 :

未共轭

反应物种 :

斑马鱼

应用 :

免疫印迹, 酶联免疫吸附测定

更多信息或购买 :

MyBioSource 产品网页

产品信息

目录号 :

MBS470008

产品类型 :

抗体

产品全称 :

C-Raf (N-terminal Region)

产品简称 :

C-Raf (N-terminal Region)

产品名称同义词 :

Raf-1

其他名称 :

c-raf; C-raf protein; RAF proto-oncogene serine/threonine-protein kinase; serine/threonine protein kinase RAF1; murine leukemia viral oncogene homolog; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene homolog 1a

其他基因名称 :

raf1a; raf1a; craf; raf1; c-raf; c-raf

UniProt数据库进入名 :

Q90458_DANRE

克隆性 :

单克隆

抗体亚型 :

IgG1

宿主 :

小鼠

反应物种 :

人类, 大鼠, 小鼠

特异性 :

This antibody detects a 74 kDa* protein corresponding to the molecular mass of C-Raf on SDS-PAGE immunoblots of human A431 and Jurkat cells.

纯度 :

蛋白质G纯化

形式 :

Mouse monoclonal, protein G purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

储存稳定性 :

Store at -20 degree C. Do not aliquot. Stable for 1 year.

检测过的应用 :

酶联免疫吸附测定, 免疫印迹

应用笔记 :

ELISA: 1:2000. Western Blot: 1:1000

其它信息1 :

Immunogen: Clone (M208) was generated from a sequence corresponding to amino acids in the N-terminal region of human C-Raf. This C-Raf sequence has high homology to rat and mouse C-Raf.

产品描述 :

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, BRaf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf (S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation.

产品引用 :

Mason, C.S. et al. (1999) EMBOJ 18(8):2137. Zhu, J. et al. (2005). Mol. Biol. Cell 16:4733.

NCBI GI登录号 :

534977

UniProt数据库登录号 :

Q90458

NCBI分子量 :

34,871 Da

NCBI信号通路 :

ARMS-mediated Activation Pathway (364821); Adaptive Immunity Signaling Pathway (365282); Androgen Receptor Signaling Pathway (198968); Axon Guidance Pathway (364570); B Cell Receptor Signaling Pathway (198947); Cytokine Signaling In Immune System Pathway (365336); Down-stream Signal Transduction Pathway (365202); EGFR1 Signaling Pathway (198963); EPO Receptor Signaling Pathway (198956); ERK1 - ERK2 MAPK Cascade Pathway (198962)

尺寸1 :

0.1毫升

价格1 :

280美元

更多信息或购买 :

MyBioSource 产品网页

公司信息